The kinetics of 3'-azido-3'-deoxythymidine phosphorylation with [32P]orthophosphoric acid was studied in the presence of various coupling agents. The most effective method with the use of BrCN provided the isolation of the target 3'-azido-3'-deoxythymidine 5'-[32P]monophosphate in 46% yield and a high specific radioactivity (>100 Ci/mmol).
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
coupling agents
8
[the study
4
study coupling
4
agents phosphorylation
4
phosphorylation 3'-azido-3'-deoxythymidine
4
3'-azido-3'-deoxythymidine p32orthophosphoric
4
p32orthophosphoric acid]
4
acid] kinetics
4
kinetics 3'-azido-3'-deoxythymidine
4
3'-azido-3'-deoxythymidine phosphorylation
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!