The yeast two-hybrid system is a powerful molecular genetic tool conceived by Fields and Song. The article is a comprehensive set of methods designed to take the reader through a yeast two-hybrid analysis of your favorite gene (YFG). This article details the preparation for a screen, the screen itself, as well as the analysis of the positives identified. Using these methods, the readers should be able to successfully negotiate a yeast two-hybrid screen using the Fields' or related systems.
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| http://dx.doi.org/10.1385/1-59259-958-3:345 | DOI Listing |
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The synergistic roles of MsRCI2B and MsRCI2E in the regulation of ion balance and ROS homeostasis in alfalfa under salt stress.
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College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:
Under salt stress, plasma membrane proteins regulate ion homeostasis and the balance between reactive oxygen species (ROS). In this study, we investigated the functions of two small membrane proteins-MsRCI2B (tailless) and MsRCI2E (tailed)-encoded by the RCI2 (Rare Cold Inducible 2) gene family in Medicago sativa (alfalfa). We identified the distinct subcellular localization and expression patterns of these proteins under salt stress.
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College of Life Science, Dezhou University, Dezhou 253023, China.
Thioredoxin z (TRX z) plays a significant role in chloroplast development by regulating the transcription of chloroplast genes. In this study, we identified a pentatricopeptide repeat (PPR) protein, rice albino seedling-lethal (RAS), that interacts with OsTRX z. This interaction was initially discovered by using a yeast two-hybrid (Y2H) screening technique and was further validated through Y2H and bimolecular fluorescence complementation (BiFC) experiments.
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View Article and Find Full Text PDFCasein Kinase I Protein Hrr25 Is Required for Pin4 Phosphorylation and Mediates Cell Wall Integrity Signaling in .
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Background: Casein kinase I protein Hrr25 plays important roles in many cellular processes, including autophagy, vesicular trafficking, ribosome biogenesis, mitochondrial biogenesis, and the DNA damage response in . Pin4 is a multi-phosphorylated protein that has been reported to be involved in the cell wall integrity (CWI) pathway and DNA damage response. Pin4 was reported to interact with Hrr25 in yeast two-hybrid and large-scale pulldown assays.
View Article and Find Full Text PDFGibberellin regulates the synthesis of stone cells in 'Nanguo' pear via the PuMYB91-PuERF023 module.
Physiol Plant
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Key Laboratory of Fruit Postharvest Biology, Liaoning Province; College of Horticulture, Shenyang Agricultural University, Shenyang, China.
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