In this paper we introduce a new storage method of PCR ingredient. PCR mixture except DNA template has been frozen to dry powder by the DNA-Plus system. This kind of powder was stored at room temperature or 4 degrees. PCR has been run in different period of storage. It was discovered that the samples of lyophilization could keep activity for a long time.
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Mol Pharm
January 2025
Laboratory of Thermodynamics, Department of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Street 70, Dortmund 44227, Germany.
Lyophilization remains a key method for preserving sensitive biopharmaceuticals such as monoclonal antibodies. Traditionally, stabilization mechanisms have been explained by vitrification, which minimizes molecular mobility in the lyophilized cake, and water replacement, which restores molecular interactions disrupted by water removal. This study proposes a novel design strategy that combines water activity and glass-transition temperature as the main indicators to predict long-term stability in lyophilized formulations.
View Article and Find Full Text PDFAdv Sci (Weinh)
December 2024
Freie Universität Berlin, Institut für Chemie und Biochemie - Anorganische Chemie, Fabeckstr. 34/36, 14195, Berlin, Germany.
Herein hyperbranched polyethyleneimine (hPEI) cryogels are reported for the selective and reversible adsorption of elemental chlorine. The cryogels are prepared in an aqueous solution by crosslinking with glutaraldehyde at subzero temperatures. The final macroporous composites bearing ammonium chloride groups are obtained after freeze-drying.
View Article and Find Full Text PDFFood Sci Nutr
December 2024
Department of Food Engineering, Faculty of Engineering Ege University İzmir Turkey.
This study investigated the survival dynamics of BG24, a probiotic strain, within reconstituted skim milk (RSM) and yeast extract (YE) matrices during the spray-drying (SD) process, encompassing of inlet/outlet air temperatures. Notably, optimum SD parameters were found to be an inlet air temperature of 150°C and outlet air temperature of 83°C, that achieving high viability (92.23%), and reducing both moisture content (MC) (3.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, Uttar Pradesh, India; Centre for Analysis, Testing, Evaluation & Reporting Services (CATERS), CSIR-Central Leather Research Institute (CSIR-CLRI), Adyar, Chennai 600020, Tamil Nadu, India.
Existing polystyrenic and polyolefinic packaging foams are non-biodegradable, and persist in the environment for hundreds of years. Gelatin foams are an interesting alternative, given their biodegradability, biocompatibility, solution-based processability, low cost, and non-toxicity. However, current methods for preparing gelatin foams, such as freeze-drying, microfluidic foaming, and batch foaming, are not suitable for high-volume production.
View Article and Find Full Text PDFEnviron Pollut
November 2024
Institute of Geology and Palaeontology - Applied Geology, University of Münster, Corrensstr. 24, 48149, Münster, Germany. Electronic address:
PAH extraction methods have been widely studied over the last decades. However, there is still no consistent method for contaminated soils. Here, for the first time, PAH source characteristics in soils were considered for the investigation of different pretreatment methods, extraction techniques and solvents in nine petrogenic to primarily pyrogenic urban soils.
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