[Sequence analysis of polymorphic fragments of porcine H-FABP gene].

Yi Chuan

Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100094 Beijing, China.

Published: March 2002

Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5'-upstream, HaeIII-RFLP and HinfI*-RFLP in intron 2, were confirmed by PCR-RFLP method. The polymorphic fragments were cloned and sequenced. The results revealed a single nucleotide substitution of T-->C at position 1324 for HinfI-RFLP,C-->G at position 1811 for HaeIII-RFLP and T-->C at position 1970 for HinfI*-RFLP, respectively.

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