There is a need for simple and inexpensive diagnostic and screening tests for the detection of Cryptosporidium parvum infection in calves. A sucrose wet mount test and a lateral immunochromatography test were evaluated for epidemiological sensitivity and specificity, cost per test, simplicity, test time and ease of batching. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the Cryptosporidium oocyst wall protein (COWP) gene locus, with gel electrophoresis, was used as a gold standard. Cohen's kappa statistic of agreement (kappa) between the Ontario Veterinary College (OVC) sucrose wet mount test and COWP PCR-RFLP was 0.82, and the sensitivity and specificity of the OVC sucrose wet mount test were 88.6% and 93.8%, respectively. The sensitivity and specificity of the lateral immunochromatography test were 78.3% and 93.3%, respectively, and agreement between this test and PCR-RFLP was good (kappa=0.73). There was substantial agreement between the OVC sucrose wet mount test and the lateral immunochromatography test (kappa=0.84). Both tests were inexpensive and easy to use; however, the lateral immunochromatography test was faster and simpler to perform than the sucrose wet mount test, and was generally more user-friendly. These tests provide practitioners and researchers with cheap, quick and accurate methods of detecting C. parvum infection in young calves.
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http://dx.doi.org/10.1016/j.vetpar.2005.06.023 | DOI Listing |
Curr Res Microb Sci
November 2024
National Technological Institute of Mexico, Conkal campus, Technological Avenue, s/n C.P. 97345, Conkal, Yucatán, Mexico.
Botanical gardens, areas for vegetation conservation, have become important reservoirs of beneficial soil microbiota, mainly as a source of microbial inoculum for agricultural purposes. Shrubby mycorrhizal fungi (AMF), an important genetic resource of tropical soils, have a high potential for agricultural production, generally used as inoculant medium that provides better yield, productivity and physiological response to crops. This research study explores the presence of AMF in a botanical garden, composed of four areas: cactarium collection, epiphytes and ornamental collection, tropical forest area and coastal zone.
View Article and Find Full Text PDFEpilepsy Behav
December 2024
Núcleo de Neurociências, Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas - Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Centro de Tecnologia e Pesquisa em Magneto Ressonância, Programa de Pós-Graduação em Engenharia Elétrica, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:
This study used intra-hippocampal injections of Kainic Acid (KA) in Wistar rats to induce spontaneous recurrent seizures (SRS) after a 9-day latent period. A post-conditioning protocol with LPS, injected at the same site 72 h after the initial KA insult, was employed to trigger secondary competing processes. To evaluate the post-conditioning effect of LPS, 25 animals were divided into four groups: SAL-SAL (n = 6), KA-SAL (n = 6), SAL-LPS (n = 7), and KA-LPS (n = 6).
View Article and Find Full Text PDFDis Model Mech
November 2024
Department of Ophthalmology, Research Laboratory of Ophthalmology and Vision Sciences, Eye Research Institute, West China Hospital, Sichuan University, Chengdu 610041, China.
Appl Physiol Nutr Metab
October 2024
Department of Kinesiology, Indiana University School of Public Health - Bloomington, Bloomington, IN, USA.
Occupational heat stress increases acute kidney injury risk. Drinking a soft drink sweetened with high fructose corn syrup further elevates this acute kidney injury risk. However, the impact of sucrose, another fructose-containing sweetener, on acute kidney injury risk remains unexplored.
View Article and Find Full Text PDFACS Omega
June 2024
Department of Engineering, University of Messina, Contrada Di Dio, I-98166 Messina, Italy.
Despite a variety of glucose sensors being available today, the development of nonenzymatic devices for the determination of this biologically relevant analyte is still of particular interest in several applicative sectors. Here, we report the development of an impedimetric, enzyme-free electrochemical glucose sensor based on carbon nanofibers (CNFs) functionalized with an aromatic diamine via a simple wet chemistry functionalization. The electrochemical performance of the chemically modified carbon-based screen-printed electrodes (SPCEs) was evaluated by electrical impedance spectroscopy (EIS), demonstrating a high selectivity of the sensor for glucose with respect to other sugars, such as fructose and sucrose.
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