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Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower.
BMC Microbiol
January 2025
USDA-ARS, Sustainable Agricultural Systems Laboratory, Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA.
Background: Pseudomonas syringae pv. tagetis (Pstag) causes apical chlorosis on sunflower and various other plants of the Asteraceae family. Whole genome sequencing of Pstag strain EB037 and transposon-mutant derivatives, no longer capable of causing apical chlorosis, was conducted to improve understanding of the molecular basis of disease caused by this pathogen.
View Article and Find Full Text PDFThe Vacuolar Inositol Transporter BvINT1;1 Contributes to Raffinose Biosynthesis and Reactive Oxygen Species Scavenging During Cold Stress in Sugar Beet.
Plant Cell Environ
January 2025
University of Kaiserslautern, Plant Physiology, Paul-Ehrlich-Str., Kaiserslautern, Germany.
Despite a high sucrose accumulation in its taproot vacuoles, sugar beet (Beta vulgaris subsp. vulgaris) is sensitive to freezing. Earlier, a taproot-specific accumulation of raffinose was shown to have beneficial effects on the freezing tolerance of the plant.
View Article and Find Full Text PDFIdentification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides.
J Immunol Methods
January 2025
Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoon-Ose, Yangzhou University, Yangzhou, China. Electronic address:
Background: Bovine IgG1 Fc receptor (boFcγRI) is a homologue to human FcγRI (CD64) that has three extracellular Ig-like domains and can bind bovine IgG1 with high affinity. Identification of the linear epitope for Fc-binding on boFcγRI provides new insights for the IgG-Fcγ interaction and FcγR-targeting drugs development.
Methods: The boFcγRI molecules were expressed on cell surface of the boFcγRI -transfected COS-7 cells.
AUK3 is required for faithful nuclear segregation in the bloodstream form of Trypanosoma brucei.
Mol Biochem Parasitol
February 2025
University of Glasgow, Centre for Parasitology, School of Infection and Immunity, 120 University Place, Sir Graeme Davies Building, Glasgow G12 8TA, United Kingdom. Electronic address:
Eukaryotic chromosomes segregate faithfully prior to nuclear division to ensure genome stability. If segregation becomes defective, the chromosome copy number of the cell may alter leading to aneuploidy and/or polyploidy, both common hallmarks of cancers. In eukaryotes, aurora kinases regulate chromosome segregation during mitosis and meiosis, but their functions in the divergent, single-celled eukaryotic pathogen Trypanosoma brucei are less understood.
View Article and Find Full Text PDFThe identification of XPR1 as a voltage- and phosphate-activated phosphate-permeable ion channel.
Res Sq
December 2024
Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA.
Maintaining a balance of inorganic phosphate (Pi) is vital for cellular functionality due to Pi's essential role in numerous biological processes. Proper phosphate levels are managed through Pi import and export, facilitated by specific Pi transport proteins. Although the mechanisms of Pi import have been extensively studied, the processes governing Pi export remain less understood.
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