Objective: To establish a rapid method for detecting Enteropathogenic Escherichia coli (EPEC) in meat and meat product.
Methods: Based on attaching and effacing lesion (eaeA gene) and bundle-forming pili (bfpA gene) of EPEC, two pairs of primers were designed. Then EPEC in meat and meat product were detected by double PCR.
Results: The method is rapid and specific. The limit of detection is 10 cfu/g in 8 - 17 hours.
Conclusion: A simple, rapid, sensitive and specific PCR method can be established to detect EPEC in meat and meat product.
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