Objective: Applying RDPCR to detect the DNA damage of N-ras gene induced by Potassium dichromate
Methods: Preparing single-stranded probes of exon 1 of N-ras gene in human. The genomic DNA was treated with Potassium dichromate, then amplified by RDPCR and detected by Southern hybridization with the probe.
Results: The clear hybridized bands can be seen in the position which is induced by Potassium dichromate on the dose of 100 micromol/L, but can't be detected on the dose over 1000 mol/L.
Conclusion: It indicates that Potassium dichromate can cause the DNA damage of N-ras gene, which should be the key point of its carcinogenesis mechanisms.
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