[Study on tissue culture and plant regeneration of Nervilia fordii].

Objective: Tissue culture of Nervilia fordii to get its regeneration.

Method: Effects on indusement of rhizoma and plant regeneration of different implants, density of hormones, additives were studied.

Result: The best implant was conn. Effect of 6-BA 2 mg x L(-1) were better than 6-BA 1 on rhizoma reducing. The coconut juice and active carbon could increase the growth of rhizoma.

Conclusion: Bud could be induced on 1/2MS + 6-BA 2 mg x L(-1) by inoculating corm on culture mediem, and could grow lots of rhizoma after inoculating on the culture mediem containing 10% coconut juice and 1 per thousand active carbon. The white rhizoma could be induced to corms and regeneration plants on 1/2MS + 1 per thousand active carbon. The green rhizoma could be induced directerly to regeneration plants on 1/2MS + 6-BA 2 + NAA 2.