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A simple approach to fabricate chitosan-delivered avermectin controlled release microparticles for improved efficacy and reduced residues.
Int J Biol Macromol
January 2025
College of Chemistry and Pharmacy, Qingdao Agricultural University, Qingdao 266109, Shandong, PR China. Electronic address:
The presence of a synergistic effect between carrier and insecticide in controlled release formulations is highly desirable to improve efficacy to target pests and reduce insecticide use. Herein, controlled release microparticles of avermectin (AVM) were fabricated using natural chitosan (CTS) as a carrier by a pH adjustment method. The resulted AVM@CTS microparticles displayed high encapsulation efficiency (73.
View Article and Find Full Text PDFStaining Tissues with Basic Blue 7: A New Dual-Polarity Matrix for MALDI Mass Spectrometry Imaging.
Anal Chem
January 2025
Department of Chemistry, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.
Obtaining high-quality matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) images and the reproducibility of the technique depend strongly on the sample preparation protocol. The most crucial part is the application of the MALDI matrix, which often relies on expensive spraying or sublimation coaters. In this work, we present a new dual-polarity matrix for MALDI mass spectrometry imaging (MSI): Basic Blue 7 (BB7), which belongs to the group of triarylmethane dyes.
View Article and Find Full Text PDFSensitive fluorescent detection of SARS-CoV-2 RNA using an enzymatic-based method.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
CICS-UBI - Health Sciences Research Centre University of Beira Interior Covilhã Portugal; RISE-Health, Departamento de Química, Faculdade de Ciências, Universidade da Beira Interior, Rua Marquês d'Ávila e Bolama 6201-001 Covilhã, Portugal; Departamento de Química, Universidade da Beira Interior, Rua Marquês de Ávila e Bolama 6201-001 Covilhã, Portugal. Electronic address:
Rapid, quantitative, and sensitive detection of viral oligonucleotides can help to diagnose the infection before symptoms occur, monitor disease progression, and identify viral subtypes. A one-pot, simple, rapid hairpin-mediated nicking enzymatic signal amplification (HNESA) method was previously developed for nucleic acids detection. In the present work, this method was applied for the detection of SARS-CoV-2 RNA by designing an assistant probe (AP) that contains the complementary sequence for the target, the sequence of hybridization with the loop region of the molecular beacon (MB), and the recognition site of the nicking endonuclease Nt.
View Article and Find Full Text PDFStudying Cationic Liposomes for Quick, Simple, and Effective Nucleic Acid Preconcentration and Isolation.
Anal Chem
January 2025
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstr. 31, Regensburg 93053, Germany.
To ensure high quality of food and water, the identification of traces of pathogens is mandatory. Rapid nucleic acid-based tests shorten traditional detection times while maintaining low detection limits. Challenging is the loss of nucleic acids during necessary purification processes, since elution off solid surfaces is not efficient.
View Article and Find Full Text PDFArthroscopic-assisted total wrist arthrodesis: surgical design and clinical outcomes.
J Plast Surg Hand Surg
January 2025
Department of Hand Surgery, Huashan Hospital Fudan University Shanghai, China; Department of Hand and Upper Extremity Surgery, Shanghai Jing'an District Central Hospital, Shanghai, China; National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Hand Reconstruction, Fudan University, Shanghai, China.
Introduction: This study presents an innovative arthroscopy-assisted total wrist arthrodesis technique utilising three hollow screws, aimed at improving clinical outcomes for patients with severe wrist arthritis.
Materials And Methods: The technique involved the placement of three hollow screws to facilitate wrist bone fusion. Between August 2019 and August 2023, four patients diagnosed with severe wrist arthritis underwent the arthroscopy-assisted procedure.
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