LEAFY COTYLEDON 2 (LEC2) is a key regulator of seed maturation in Arabidopsis. To unravel some of its complex pleiotropic functions, analyses were performed with transgenic plants expressing an inducible LEC2:GR protein. The chimeric protein is functional and can complement lec2 mutation. Interestingly, the induction of LEC2 leads to the accumulation of storage oil in leaves. In addition, short-term induction and use of translation inhibitors allowed to demonstrate that LEC2 can directly trigger the accumulation of seed specific mRNAs. Consistent with these results, the expression of three other major seed regulators namely, LEC1, FUS3, and ABI3 were also induced by LEC2 activation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.febslet.2005.07.037 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
AtLEC2-Mediated Enhancement of Endoplasmic Reticulum-Targeted Foreign Protein Synthesis in Nicotiana benthamiana Leaves: Insights From Transcriptomic Analysis.
Biotechnol Bioeng
November 2024
Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDCA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Many proteins used in industrial and pharmaceutical applications are typically synthesized within the secretory pathway. While yeast and mammalian cells have been engineered to enhance the production of endomembrane-targeted proteins, similar strategies in plant cells remain underexplored. This study investigates the potential of arabidopsis leafy cotyledon 2 (AtLEC2), a key regulator of seed development, to enhance the production of proteins targeted to the endoplasmic reticulum (ER) in Nicotiana benthamiana leaves.
View Article and Find Full Text PDFFunctional Analysis of the PoSERK-Interacting Protein PorbcL in the Embryogenic Callus Formation of Tree Peony ( T. Hong et J. X. Zhang).
Plants (Basel)
September 2024
College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China.
is a marker gene for early somatic embryogenesis. We screened and functionally verified a SERK-interacting protein to gain insights into tree-peony somatic embryogenesis. Using PoSERK as bait, we identified PorbcL (i.
View Article and Find Full Text PDFOsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo.
Elife
September 2024
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/ Zhongshan Biological Breeding Laboratory, Agricultural College of Yangzhou University, Yangzhou, China.
As a master regulator of seed development, Leafy Cotyledon 1 (LEC1) promotes chlorophyll (Chl) biosynthesis in , but the mechanism underlying this remains poorly understood. Here, we found that loss of function of , a homolog of rice, leads to chlorophyllous embryo, indicating that plays an opposite role in Chl biosynthesis in rice compared with that in . OsNF-YB7 regulates the expression of a group of genes responsible for Chl biosynthesis and photosynthesis by directly binding to their promoters.
View Article and Find Full Text PDFmiR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways.
Int J Mol Sci
August 2024
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, 40-007 Katowice, Poland.
The embryogenic transition of plant somatic cells to produce somatic embryos requires extensive reprogramming of the cell transcriptome. The prominent role of transcription factors (TFs) and miRNAs in controlling somatic embryogenesis (SE) induction in plants was documented. The profiling of expression in the embryogenic culture of Arabidopsis implied the contribution of the miR156 and miR169 to the embryogenic induction.
View Article and Find Full Text PDFPeanut LEAFY COTYLEDON1-type genes participate in regulating the embryo development and the accumulation of storage lipids.
Plant Cell Rep
April 2024
Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Ji'nan, 250100, Shandong Province, China.
Two peanut LEC1-type genes exhibit partial functional redundancy. AhNFYB10 could complement almost all the defective phenotypes of lec1-2 in terms of embryonic morphology, while AhNF-YB1 could partially affect these phenotypes. LEAFY COTYLEDON1 (LEC1) is a member of the nuclear factor Y (NF-Y) family of transcription factors and has been identified as a key regulator of embryonic development.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!