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Multicellular structures developing during maize microspore culture express endosperm and embryo-specific genes and show different embryogenic potentialities. | LitMetric

AI Article Synopsis

  • - During maize pollen embryogenesis, various multicellular structures with different embryogenic potentials were identified, including embryo-like structures (ELS) and callus-like structures (CLS) that appeared temporarily in cultures.
  • - Advanced techniques like RT-PCR and fluorescence in situ hybridization (FISH) revealed distinct patterns of gene expression associated with these structures, highlighting their potential to develop into embryos or endosperm.
  • - The findings suggest significant interactions between the embryonic and non-embryonic structures, drawing parallels to how embryo and endosperm function together in plants under natural conditions.

Article Abstract

During maize pollen embryogenesis, a range of multicellular structures are formed. Using different approaches, the "nature" of these structures has been determined in terms of their embryogenic potential. In situ molecular identification techniques for gene transcripts and products, and a novel cell tracking system indicated the presence of embryogenic (embryo-like structures, ELS) and non-embryogenic (callus-like structures, CLS) structures that occurred for short periods within the cultures. Some multicellular structures with a compact appearance generated embryos. RT-PCR and fluorescence in situ hybridization (FISH) with confocal microscopy techniques using specific gene markers of the endosperm (ZmESR2, ZmAE3) and embryo (LTP2 and ZmOCL1, ZmOCL3) revealed "embryo" and "endosperm" potentialities in these various multicellular structures present in the cultures. The results presented here showed distinct and specific patterns of gene expression. Altogether, the results demonstrate the presence of different molecules on both embryonic and non-embryonic structures. Their possible roles are discussed in the context of a parallel between embryo/endosperm interactions in planta and embryonic and non-embryonic structure interrelations under in vitro conditions.

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Source
http://dx.doi.org/10.1016/j.ejcb.2005.02.002DOI Listing

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