AI Article Synopsis

  • A new gas chromatography-high-resolution mass spectrometric (GC-HRMS) method is developed for accurately detecting methamphetamine (MA) and its main metabolite, amphetamine (AMP), in human hair samples.
  • The procedure includes steps like decontaminating the hair, hydrolysis, extraction, and analysis using selective ion monitoring, achieving notable limits of detection at 9 pg/mg for MA and 21 pg/mg for AMP.
  • This method also shows improved sensitivity and results over conventional techniques, with high recovery rates and successful application to challenging samples like cosmetically treated hair.

Article Abstract

Gas chromatography-high-resolution mass spectrometric (GC-HRMS) method is presented for the qualitative and quantitative analysis of methamphetamine (MA) and its major metabolite, amphetamine (AMP), in human hair. The method procedure involves decontamination of hair with distilled water and acetone, acidic hydrolysis and extraction in the presence of the internal standard, and GC-HRMS selective ion monitoring (SIM) analysis. The limits of detection (LOD) were 9 pg/mg for MA and 21 pg/mg for AMP using a 30-mg hair sample, and the SIM responses were linear with coefficients of correlation ranged from 0.9998 to 0.9999. The recoveries were found to be 91.1-92.3%. By using HRMS (resolution of 5000), detection sensitivity is improved because of the elimination of the biological background, and the LODs for MA and AMP were 2.4-4.4 times lower than those of low-resolution MS. The GC-HRMS method was successfully applied to the analysis of cosmetically treated hair, which is difficult to analyze with the conventional method.

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Source
http://dx.doi.org/10.1093/jat/29.5.370DOI Listing

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