Objective: To study the chemical constituents from the methanolic extract from the leaf of Liquidambar formosana Hance.

Method: The compounds were separated with the column chromatographic and Prep HPLC, and their structures were identified by chemical and spectroscopic methods.

Results: Three compounds were isolated from the methanolic extract. They were identified as: beta-sitosterol (I), alpha-amyrin (II) and oleanonic acid (III).

Conclusion: All of them were isolated from this plant for the first time.

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