AI Article Synopsis
- Centrins are calcium-binding proteins part of the CaM superfamily, specifically from the ciliate Euplotes Octocarinatus.
- The centrin was expressed in E. coli, purified, and crystallized using the hanging-drop method.
- The resulting rod-like crystals diffracted to 2.0 angstroms and belong to the space group P2(1)2(1)2(1) with specific unit-cell parameters.
Article Abstract
Centrins are four-EF-hand Ca(2+)-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstroms. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstroms, b=48.954 angstroms, c=72.583 angstroms.
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| http://dx.doi.org/10.2174/0929866054395842 | DOI Listing |
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