AI Article Synopsis
- Hydrodynamic delivery is a promising method for safely delivering DNA and other molecules directly into cells via injection into the bloodstream, avoiding risks associated with viral and synthetic delivery systems.
- This technique uses high-pressure injection of a large volume of fluid to help the DNA bypass barriers like cell membranes and reach target cells in various tissues.
- The review covers the evolution of hydrodynamic delivery, how it's currently applied, and its potential future applications in clinical settings.
Article Abstract
Hydrodynamic delivery has emerged as a near-perfect method for intracellular DNA delivery in vivo. For gene delivery to parenchymal cells, only essential DNA sequences need to be injected via a selected blood vessel, eliminating safety concerns associated with current viral and synthetic vectors. When injected into the bloodstream, DNA is capable of reaching cells in the different tissues accessible to the blood. Hydrodynamic delivery employs the force generated by the rapid injection of a large volume of solution into the incompressible blood in the circulation to overcome the physical barriers of endothelium and cell membranes that prevent large and membrane-impermeable compounds from entering parenchymal cells. In addition to the delivery of DNA, this method is useful for the efficient intracellular delivery of RNA, proteins, and other small compounds in vivo. This review discusses the development, current application, and clinical potential of hydrodynamic delivery.
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| http://dx.doi.org/10.1016/S0065-2660(05)54004-5 | DOI Listing |
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