AI Article Synopsis
- Establishing a reliable genotyping protocol is crucial for post-sequence genetics, and this article presents a sensitive method for detecting DNA by using molecular-scale DNA probes.
- The electrochemical detection method is highly effective, showing significant changes in response with even a single base mismatch in the DNA sequence.
- This technique successfully identifies various single nucleotide polymorphisms (SNPs) and can differentiate between wild-type DNA and specific genetic variations in the p53 gene.
Article Abstract
Establishing a reliable genotyping protocol is a critical matter in post-sequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183446 | PMC |
| http://dx.doi.org/10.1073/pnas.0502078102 | DOI Listing |
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