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Use of a heminested reverse transcriptase PCR assay for detection of astrovirus in environmental swabs from an outbreak of gastroenteritis in a pediatric primary immunodeficiency unit. | LitMetric

AI Article Synopsis

  • An astrovirus gastroenteritis outbreak in March 2004 at Newcastle General Hospital's Primary Immunodeficiency Unit led to environmental swabbing to identify contamination.* -
  • Detection of the astrovirus genotype 3 strain was confirmed in both environmental swabs and patient fecal samples, linking these sources to the outbreak.* -
  • The virus likely spread through a contaminated syringe pump, highlighting the importance of environmental monitoring in hospitals to prevent future infections and ensure effective cleaning protocols.*

Article Abstract

An outbreak of astrovirus gastroenteritis occurred in the Primary Immunodeficiency Unit at Newcastle General Hospital in March 2004. Environmental swabbing of the unit was undertaken after the outbreak, with multiple sites swabbed pre- and postcleaning. Astroviruses were detected in four environmental swabs and from two patient fecal samples using heminested reverse transcriptase PCR. An astrovirus genotype 3 strain was identified in both environmental swabs and fecal specimens and was the strain identified as being responsible for the outbreak. Environmental transmission of the virus was thought to have occurred by contamination of a syringe pump outside the laminar-flow curtain of a patient who was admitted with astrovirus gastroenteritis. This was subsequently transmitted to a cubicle next door and to a television/games console in a parents' room in the ward. Environmental monitoring of surfaces/equipment, using PCR assays for gastroenteric viruses in hospital situations where infection can give rise to serious clinical complications, may have a role in controlling and monitoring cleaning and the subsequent prevention of nosocomial transmission of gastroenteritis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1234003PMC
http://dx.doi.org/10.1128/JCM.43.8.3890-3894.2005DOI Listing

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