AI Article Synopsis
- The study characterized the AM fungus Scutellospora reticulata using a mix of morphological, ontogenetic, and molecular methods, noting an unusual pattern in its spore wall development.
- The research employed Denaturing Gradient Gel Electrophoresis (DGGE) to distinguish S. reticulata from 16 other Scutellospora species, confirming findings with sequencing analysis.
- Phylogenetic results positioned S. reticulata closely with S. cerradensis and S. heterogama, indicating shared features like similar spore wall structures, and showed that intragenomic SSU nrDNA polymorphisms could effectively differentiate related Scutellospora species.
Article Abstract
The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.
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| http://dx.doi.org/10.1017/s0953756205002546 | DOI Listing |
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