Amylase secretion from rat parotid acinar cells is induced by the accumulation of cAMP in response to beta-adrenergic agonists as well as by the elevation of intracellular Ca2+ in response to muscarinic cholinergic stimulation. Several proteins including the low molecular weight GTP-binding protein Rab may participate in these exocytic processes. In the current studies, we investigated the role of Rab26 in the process of amylase secretion. Secretory granules were separated by centrifugation on a Percoll-sucrose density gradient into mature and immature granule fractions. Rab26 and two other type III Rab proteins, Rab3D and Rab27, were present in the mature granule membrane fraction. Also, Rab26 was absent in immature granule membrane fractions. Isoproterenol-induced amylase release from streptolysin-O-permeabilised acinar cells was inhibited by an anti-Rab26 antibody, but this antibody had no effect on the Ca2+-induced release of amylase. Finally, in the early stage of beta-adrenergic stimulation, Rab26 was condensed in the secretory granule membrane. These results indicate that Rab26 is involved in the recruitment of mature granules to the plasma membrane upon beta-adrenergic stimulation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.archoralbio.2005.06.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spontaneous Transformation from Lung Adenocarcinoma to MYC-amplified Large Cell Neuroendocrine Carcinoma.
Pathol Int
January 2025
Department of Cancer Pathology, Graduate School of Medicine, Hokkaido University, Hokkaido, Japan.
Recent studies suggest that lung adenocarcinoma cells are closely associated with the tumorigenesis of large-cell neuroendocrine carcinoma via cellular transformation. However, morphological evidence, along with genetic abnormalities before, during, and after transformation, is quite limited. We present here a case of combined large-cell neuroendocrine carcinoma and adenocarcinoma exhibiting acinar and solid patterns.
View Article and Find Full Text PDFExtracellular and intracellular digestion in the bivalves studied by magnetic resonance imaging with a contrast reagent.
J Exp Biol
January 2025
Division of Cell Structure, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
We investigated the extracellular and intracellular digestion of bivalves employing magnetic resonance imaging (MRI). Ruditapes philippinarum clams and Mytilus galloprovincialis mussels were incubated in seawater containing a contrast reagent (GdDTPA) at 20°C. The digestive systems, from the esophagus to the rectum, were visualized at a high signal intensity by the T1-weighted MRI.
View Article and Find Full Text PDFTotal saponin extracts of Pseudostellaria heterophylla ameliorates meibomian gland dysfunction through SCD1/SPT1/ceramide axis.
J Ethnopharmacol
January 2025
The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine, Xiamen University, Xiamen, China; Xiamen Key Laboratory of Chiral Drugs, Xiamen, China. Electronic address:
Ethnopharmacological Relevance: Pseudostellaria heterophylla (Tài Zǐ Shēn, TZS) is a traditional Chinese medicine with spleen and qi benefits. Its immunomodulatory, anti-fatigue, anti-stress, and lipid metabolism regulation effects have been clinically confirmed, but its role in meibomian gland dysfunction (MGD) is still unclear.
Aim Of The Study: This study aims to investigate the effect and mechanism of action of TZS in treating MGD.
Genetic and pharmacological targeting of HINT2 promotes OXPHOS to alleviate inflammatory responses and cell necrosis in acute pancreatitis.
Pharmacol Res
January 2025
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, Chengdu, China. Electronic address:
The necrosis of pancreatic acinar cells is a key molecular event in the progression of acute pancreatitis (AP), with disturbances in mitochondrial energy metabolism considered to be a direct causative factor of acinar cell necrosis. Histidine triad nucleotide-binding protein 2 (HINT2) has been implicated in the development of various diseases, whereas its involvement in the progression of AP remains unclear. This study aims to investigate the role of HINT2 in AP.
View Article and Find Full Text PDFA lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans.
Sci Rep
January 2025
Department of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research Institute, Duarte, USA.
Lectins are produced in almost all life forms, can interact with targets (glycans) in a cross-kingdom manner and have served as valuable tools for studying glycobiology. Previously, a bacterial lectin, named Streptomyces hemagglutinin (SHA), was found to agglutinate human type B erythrocytes. However, the binding of SHA to mammalian cell types other than human erythrocytes has not been explored.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!