A novel gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed, using an ion trap mass spectrometer, for the simultaneous determination of olive oil bioactive components, elenolic acid, hydroxytyrosol, and tyrosol, in rat urine. Samples were analyzed by GC-MS/MS prior to and after enzymatic treatment. A solid phase extraction sample pretreatment step with greater than 80% analytical recoveries for all compounds was performed followed by a derivatization reaction prior to GC-MS/MS analysis. The calibration curves were linear for all compounds studied for a dynamic range between 1 and 500 ng. The limit of detection was in the mid picogram level for tyrosol and elenolic acid (300 pg) and in the low picogram level for hydroxytyrosol (2.5 pg). The method was applied to the analysis of rat urine samples after sustained oral intake of oleuropein or extra virgin olive oil as a diet supplement.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf050851w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the chemical profiles and biological activities of Thesium bertramii Azn. Extracts using a combination of in vitro, in silico, and network pharmacology methods.
Fitoterapia
January 2025
Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, 63290, Turkey.
The genus Thesium, family Santalaceae, comprises about 350 species, and, although many of them are used as functional food and in traditional medicine, there are limited studies evaluating their pharmacological potential. The present study was designed to evaluate the chemical profile, antioxidant, and enzyme inhibition potential of aerial parts and roots of T. bertramii Azn.
View Article and Find Full Text PDFMonitoring the Phenolic and Terpenic Profile of Olives, Olive Oils and By-Products throughout the Production Process.
Foods
May 2024
Department of Food Science and Nutrition, Faculty of Farmacy, University of Granada, Campus Universitario Cartuja s/n, 18071 Granada, Spain.
Olive oil is a food of great importance in the Mediterranean diet and culture. However, during its production, the olive oil industry generates a large amount of waste by-products that can be an important source of bioactive compounds, such as phenolic compounds and terpenes, revalorizing them in the context of the circular economy. Therefore, it is of great interest to study the distribution and abundance of these bioactive compounds in the different by-products.
View Article and Find Full Text PDFNovel procedures for olive leaves extracts processing: Selective isolation of oleuropein and elenolic acid.
Food Chem
July 2024
Dipartimento di Farmacia, Università "G. d'Annunzio" Chieti - Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy. Electronic address:
Several processes have been developed in the past to selectively extract oleuropein and its aglycones from olive derived materials. In the present manuscript, we outline a novel approach for processing olive leaves aqueous extracts. This allowed first to select microwave irradiation as the methodology able to provide a large enrichment in oleuropein.
View Article and Find Full Text PDFComparative metabolomic profiling and nutritional chemistry of of diverse panicle architecture and agroecological zones.
Physiol Mol Biol Plants
December 2023
Department of Plant Pathology, University of Agriculture, University Road, Faisalabad, Pakistan.
Unlabelled: possesses remarkable nutritional value and adaptability to various agroecological conditions. Panicle architecture influences the number of spikelets and grains in a panicle, ultimately leading to productivity and yield. Therefore, this study aimed to investigate the metabolites, nutrients, and minerals in accessions of varying panicle architecture.
View Article and Find Full Text PDFNuclear magnetic resonance analysis of extra virgin olive oil: classification through secoiridoids.
J Sci Food Agric
March 2024
Laboratory of Food Chemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
Background: Extra virgin olive oil (EVOO), a natural product with a multidisciplinary role, has been and is continuing to be studied from several points of view. Among them, its chemical analysis is of major importance and several methods have been used. Nuclear magnetic resonance (NMR) spectroscopy has inherent advantages, among them monitoring the chemical constituents without the need for a separation technique and without, for instance, possible carry-over effects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!