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Is Bacillus cytotoxicus from edible insects a threat?
Int J Food Microbiol
February 2025
Department of Comparative Biomedicine and Food Science, University of Padua, Viale Università 16, 35020 Legnaro, Pd, Italy.
Bacillus cytotoxicus is considered a potential emerging foodborne pathogen that has been under investigation in recent years. Most studies have focused on strains from vegetables, particularly potato products, but there is limited information on strains from other food sources. This study addresses the current research gap by investigating the genomic and phenotypic features of B.
View Article and Find Full Text PDFCanine uropathogenic and avian pathogenic Escherichia coli harboring conjugative plasmids exhibit augmented growth and exopolysaccharide production in response to Enterococcus faecalis.
PLoS One
November 2024
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States of America.
Uropathogenic Escherichia coli (UPEC) and avian pathogenic Escherichia coli (APEC) are extraintestinal pathogenic Escherichia coli (ExPEC) that infect dogs and poultry. These agents occur both as single-species infections and, commonly, in co-infection with Enterococcus faecalis (EF); however, it is unclear how EF co-infections modulate ExPEC virulence. Genetic drivers of interspecies interactions affecting virulence were identified using macrocolony co-culture, chicken embryo co-infection experiments, and whole-genome sequence analysis of ExPEC and EF clinical isolates.
View Article and Find Full Text PDFPolymicrobial interactions between and promote biofilm formation and persistence in chronic wound infections.
bioRxiv
November 2024
Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, United States of America.
Chronic, non-healing wounds are a leading cause of prolonged patient morbidity and mortality due to biofilm- associated, polymicrobial infections. and are the most frequently co-isolated pathogens from chronic wound infections. Competitive interactions between these pathogens contribute to enhanced virulence, persistence, and antimicrobial tolerance.
View Article and Find Full Text PDFCarrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.
J Proteome Res
November 2024
Department of Biology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.
Many shotgun proteomics experiments are negatively influenced by highly abundant proteins, such as those measuring residual host cell proteins (HCP) amidst highly abundant recombinant biotherapeutic or plasma proteins amidst albumin and immunoglobulins. While western blotting and ELISAs can reveal the presence of specific low abundance proteins from highly abundant background proteins, mass spectrometry approaches are required to define the low abundance protein composition in these scenarios. The challenge in detecting low abundance proteins in a high protein background by standard shotgun approaches is that spectra are often not triggered on their peptides in data dependent acquisition methods but rather on the highly abundant background peptides.
View Article and Find Full Text PDFCircularly assembled genome sequences of sp. strain MM231 and MM232, isolated from a pond at Bielefeld University campus.
Microbiol Resour Announc
November 2024
Computational Biology, Faculty of Biology, Bielefeld University, Bielefeld, Germany.
Photosynthetic organisms often interact with heterotrophic microbes. We here report the complete genome sequences of the bacterial strains sp. MM231 and MM232.
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