The expression of prophenoloxidase (proPO) transcript in the tissues of the Pacific white shrimp Litopenaeus vannamei was studied by Northern blot, RT-PCR and in situ hybridisation. Northern blot analysis reveals that proPO was constitutive mainly in shrimp haemocytes. Its transcripts, when detected with RT-PCR, were widely distributed in haemocytes, gill, heart, lymphoid organ, stomach, midgut, anterior midgut caecum and ganglion. A lower expression was found in hepatopancreas, muscle and cuticular epidermis. The results of in situ hybridisations showed that gill, heart, muscle, haematopoitic tissue and hepatopancreas that were infiltrated by haemocytes also presented positive signals. ProPO transcripts, however, were detected in non-haemocyte cells, including F and E cells of the hepatopancreas, epithelium in stomach, anterior midgut caecum, glia cell in nervous cord and neurosecretory cells in ganglions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2005.05.003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Associations of prenatal maternal psychosocial stress and depression with neurodevelopmental outcomes in 7.5-month-old infants in the ECHO.CA.IL prospective birth cohorts.
Neurotoxicol Teratol
January 2025
University of Illinois Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 N. Mathews Ave., Urbana, IL 61821, USA. Electronic address:
Background: Exposure to maternal stress and depression during pregnancy can have a marked impact on birth outcomes and child development, escalating the likelihood of preterm birth, lower birth weight, and various domains of physical and neurodevelopment.
Methods: The joint ECHO.CA.
Durvalumab with or without bevacizumab with transarterial chemoembolisation in hepatocellular carcinoma (EMERALD-1): a multiregional, randomised, double-blind, placebo-controlled, phase 3 study.
Lancet
January 2025
Department of Diagnostic and Interventional Radiology, University of Pisa School of Medicine, Pisa, Italy.
Background: Transarterial chemoembolisation (TACE) is standard of care for patients with unresectable hepatocellular carcinoma that is amenable to embolisation; however, median progression-free survival is still approximately 7 months. We aimed to assess whether adding durvalumab, with or without bevacizumab, might improve progression-free survival.
Methods: In this multiregional, randomised, double-blind, placebo-controlled, phase 3 study (EMERALD-1), adults aged 18 years or older with unresectable hepatocellular carcinoma amenable to embolisation, an Eastern Cooperative Oncology Group performance status of 0 or 1 at enrolment, and at least one measurable intrahepatic lesion per modified Response Evaluation Criteria in Solid Tumours (RECIST) were enrolled at 157 medical sites including research centres and general and specialist hospitals in 18 countries.
Consistency in Self-Reported Race-and-Ethnicity Over Time: Implications for Improving the Accuracy of Imputations and Making the Best Use of Self-Report.
Med Care
February 2025
RAND, Health Care, Santa Monica, CA.
Background: Medicare Bayesian Improved Surname and Geocoding (MBISG), which augments an imperfect race-and-ethnicity administrative variable to estimate probabilities that people would self-identify as being in each of 6 mutually exclusive racial-and-ethnic groups, performs very well for Asian American and Native Hawaiian/Pacific Islander (AA&NHPI), Black, Hispanic, and White race-and-ethnicity, somewhat less well for American Indian/Alaska Native (AI/AN), and much less well for Multiracial race-and-ethnicity.
Objectives: To assess whether temporal inconsistency of self-reported race-and-ethnicity might limit improvements in approaches like MBISG.
Methods: Using the Medicare Health Outcomes Survey (HOS) baseline (2013-2018) and 2-year follow-up data (2015-2020), we evaluate the consistency of self-reported race-and-ethnicity coded 2 ways: the 6 mutually exclusive MBISG categories and individual endorsements of each racial-and-ethnic group.
Baseline Estimates for Companion Animals Living in Households in Hawai'i: Associated Socio-Demographic, and Select Health Variables, as Measured by a Household Survey.
Hawaii J Health Soc Welf
January 2025
Retired, Research Statistician Hawai'i Department of Health, Adjunct Assistant Professor, University of Hawai'i, Office of Public Health Studies, Honolulu, HI.
Information on companion animals in Hawai'i is lacking. The Hawai'i Department of Health's Hawai'i Health Survey, collected data on adults and households by telephone interview. National estimates of companion animals range from 50-67%.
View Article and Find Full Text PDFGenomic insights into fibrinogen-related proteins and expression analysis in the Pacific white shrimp, Litopenaeusvannamei.
Fish Shellfish Immunol
January 2025
Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266071, China. Electronic address:
Fibrinogen-related domain (FReD) containing proteins are an evolutionarily conserved immune gene family characterized by the C-terminal fibrinogen (FBG) and diverse N-terminal domains. To understand the complexity of this family in crustaceans, we performed genome screening and identified 43 full-length FReDs encoding genes in Litopenaeus vannamei. Structural classification analysis revealed these putative FReDs could be divided into six types, including two reported types (LvFReDI and II) and four new types (LvFReDIII-VI).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!