Cytochrome P450(BM3), from Bacillus megaterium, catalyses the epoxidation of linolenic acid yielding 15,16-epoxyoctadeca-9,12-dienoic acid with complete regio- and moderate enantio-selectivity (60% ee). The absolute configuration of the product is tentatively assigned as 15(R),16(S)-. The Michaelis-Menten parameters kcat and Km for the reaction were determined to be 3126 +/- 226 min(-1) and 24 +/- 6 microM respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/b506155e | DOI Listing |
Publication Analysis
Top Keywords
epoxidation linolenic
8
linolenic acid
8
cytochrome p450bm3
8
p450bm3 bacillus
8
bacillus megaterium
8
enantioselective epoxidation
4
acid catalysed
4
catalysed cytochrome
4
megaterium cytochrome
4
megaterium catalyses
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!