AI Article Synopsis

  • New sex pheromone plasmids in Enterococcus faecalis were identified, including pIP964 (haemolysin-bacteriocin), pIP1017, pIP1438, pIP1440 (R plasmids), and pIP1141, pMV120 (cryptic conjugative plasmids).
  • The identification relied on observing cell aggregates during conjugation, successful transfer in broth, and clumping reactions of plasmid-carrying cells.
  • These plasmids were shown to hybridize with DNA probes for genes that encode surface proteins necessary for their conjugative transfer.

Article Abstract

We describe the identification of the following new sex pheromone plasmids in Enterococcus faecalis: a haemolysin-bacteriocin plasmid, pIP964; three R plasmids, pIP1017, pIP1438 and pIP1440; and two cryptic conjugative plasmids, pIP1141 and pMV120. The identification was based on the formation of cell aggregates on filter membranes during conjugation, on efficient transfer in broth matings, and on a positive clumping reaction of cells carrying these plasmids. In addition these plasmids hybridized with DNA probes specific for sex pheromone-induced structural genes encoding surface proteins required for conjugative transfer of the plasmids.

Download full-text PDF

Source
http://dx.doi.org/10.1007/BF00587574DOI Listing

Publication Analysis

Top Keywords

identification sex
8
sex pheromone
8
pheromone plasmids
8
plasmids enterococcus
8
enterococcus faecalis
8
plasmids
7
faecalis describe
4
describe identification
4
faecalis haemolysin-bacteriocin
4
haemolysin-bacteriocin plasmid
4

Similar Publications

Introduction: Athletes with concussions experience heterogeneous symptoms and clinical trajectories. Subclassification provides diagnostic clarity that may improve prognostication and individualized treatments.

Methods: We hypothesized that endophenotypes of adolescent athletes with concussions differ based on sex and time since injury.

View Article and Find Full Text PDF

Background: Postoperative delirium (POD) is a common complication after major surgery and is associated with poor outcomes in older adults. Early identification of patients at high risk of POD can enable targeted prevention efforts. However, existing POD prediction models require inpatient data collected during the hospital stay, which delays predictions and limits scalability.

View Article and Find Full Text PDF

Biomarkers.

Alzheimers Dement

December 2024

The Mind Research Network, Albuquerque, NM, USA.

Background: In recent efforts to improve early identification, staging, and prediction of risk of persons at risk for vascular contributions to cognitive impairment and dementia (VCID) in relation with small vessel disease (SVD), the MarkVCID consortium has worked to identify and validate fluid- and imaging-based biomarkers for SVD associated with VCID. Free water (FW) measured derived from diffusion tensor imaging and one of the selected neuroimaging biomarker "kits", has been demonstrated to have excellent instrumental validity and to be a sensitive biomarker of cognitive performances. We sought to further examine FW clinical relevance by investigating whether FW predicts cognitive worsening over time.

View Article and Find Full Text PDF

Biomarkers.

Alzheimers Dement

December 2024

Neurophysiology & Behaviour Lab, University of Castilla-La Mancha, Ciudad Real, Spain.

Background: A key neuropathological feature in the early stages of Alzheimer's disease (AD) involves hippocampal dysfunction arising from the accumulation of amyloid-β (Aβ). Previously, our laboratory identified a shift in the synaptic plasticity long term potentiation (LTP)/long term depression (LTD) induction threshold, leading to memory deficits in a non-transgenic murine model of early AD generated by intracerebroventricular (icv.) injections Aβ oligomers (oAβ), one of the most predominant pathogenetic factors in initial stages of the disease.

View Article and Find Full Text PDF

Background: Olfactory deficits are predictive of cognitive decline and dementia. Previous studies have linked brain magnetic resonance imaging markers of neurodegeneration to olfactory deficits in aging; however, these studies analyzed cross-sectional data for markers, olfaction, or both. Furthermore, potential cerebrovascular contributions to understanding why olfactory deficits predict dementia remain unexplored.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!