Inhibition of enzyme activities and the antiwrinkle effect of polyphenol isolated from the persimmon leaf (Diospyros kaki folium) on human skin.

Background: Many studies have been conducted to find a natural material that has high biologic functions for human skin without any side effects. Persimmon leaf has a substantial amount of tannins in different forms; therefore, it was selected as a target material.

Objective: The biosynthesis rate of the collagen was also investigated to clarify the beneficial functions for the human skin.

Methods: Persimmon leaves were obtained, extracted with 80% ethanol, and isolated into PFs I, II, and III after column chromatography using a Sephadex LH-20 column followed by thin-layer chromatography.

Results: The xanthine oxidase inhibition effect of both PFs II and III was over 40% at a 100 ppm concentration. PF II, containing higher flavonoids levels, had a significantly higher tyrosinase inhibition than that of PF III. Collagenase inhibition was 16.3 and 8.1% for PF III and PF II, respectively, at 100 ppm. On the other hand, elastase inhibition activity was significantly higher in PF II than PF III. Collagen biosynthesis rates of PF III were over 25% from a 1 to 10 ppm concentration. Consequently, PFs isolated from the persimmon leaf can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the antiwrinkle effect and the inhibition of skin problems, for food or cosmetic compositions.