Purpose: To examine membrane repair mechanisms in rabbit corneal epithelial (RCE) cells.
Methods: Microneedle puncture and fluorescent dye loss were used to wound membranes and assay resealing, respectively. Different repair mechanisms were detected pharmacologically and with antisense oligonucleotides.
Results: The RCE cells rapidly reseal plasma membranes by calcium-dependent exocytotic mechanisms that exhibit both facilitated and potentiated responses to multiple wounding. The facilitated response was inhibited by specific inhibitors of protein kinase C (PKC) and brefeldin A, and the potentiated response was blocked by inhibitors of cAMP-dependent protein kinase (PKA). Reduction of myosin IIA inhibited the facilitated response, and reduction of IIB inhibited the initial resealing.
Conclusions: RCE cells rapidly repair plasma membrane disruptions. At a second wound at the same site, PKC stimulated vesicle formation from the Golgi apparatus, resulting in more rapid membrane resealing for a facilitated response. The RCE cell also contains a PKA-dependent global potentiation of membrane resealing.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/02713680590968574 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos.
Sci Rep
November 2024
Department of Obstetrics, Gynaecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
This study introduces a low-voltage electroporation microchip designed for transfection in cat embryos, featuring real-time impedance monitoring. The microchip uses a field constriction strategy, which localises the electric field to the membrane region in contact with the micro-orifice, enhancing electroporation efficiency while minimising damage. Embryos were positioned on the orifice, and a series of voltage pulses (10, 15, and 20 V) were applied.
View Article and Find Full Text PDFFish Oil Supplement Mitigates Muscle Injury In Vivo and In Vitro: A Preliminary Report.
Nutrients
October 2024
Department of Molecular & Cellular Biochemistry, University of Kentucky, 741 South Limestone Street, BBSRB 143, Lexington, KY 40536, USA.
Following injury, older adults exhibit slow recovery of muscle function. Age-related impairment of sarcolemmal membrane repair may contribute to myocyte death, increasing the need for myogenesis and prolonging recovery. Dietary fish oil (FO) is a common nutritional supplement that may alter plasma membrane composition to enhance the response to membrane injury.
View Article and Find Full Text PDFElectroporation in Cancer Therapy: A Simplified Model Derived from the Hodgkin-Huxley Model.
Bioelectricity
September 2024
Department of Electrical Engineering and Industrial Computing, Ecole Nationale Supérieure des Technologies Avancées, Algiers, Algeria.
Cancer remains a global health challenge, necessitating effective treatments with fewer side effects. Traditional methods such as chemotherapy and surgery often have complications. Pulsed electric fields and electroporation have emerged as promising approaches to mitigate these challenges.
View Article and Find Full Text PDFThe molecular impact of sonoporation: A transcriptomic analysis of gene regulation profile.
Ultrason Sonochem
December 2024
Schlegel Research Institute for Aging and Department of Electrical & Computer Engineering, University of Waterloo, Waterloo, ON N2L3G1, Canada. Electronic address:
Sonoporation has long been known to disrupt intracellular signaling, yet the involved molecules and pathways have not been identified with clarity. In this study, we employed whole transcriptome shotgun sequencing (RNA-seq) to profile sonoporation-induced gene responses after membrane resealing has taken place. Sonoporation was achieved by microbubble-mediated ultrasound (MB-US) exposure in the form of 1 MHz ultrasound pulsing (0.
View Article and Find Full Text PDFMembrane Tension Regulation is Required for Wound Repair.
Adv Sci (Weinh)
December 2024
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation (ZMBE), Multiscale Imaging Centre, Cells in Motion Interfaculty Center, University of Münster, 48149, Münster, Germany.
Disruptions of the eukaryotic plasma membrane due to chemical and mechanical challenges are frequent and detrimental and thus need to be repaired to maintain proper cell function and avoid cell death. However, the cellular mechanisms involved in wound resealing and restoration of homeostasis are diverse and contended. Here, it is shown that clathrin-mediated endocytosis is induced at later stages of plasma membrane wound repair following the actual resealing of the wound.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!