Objective: To investigate the inhibitory effects of Snail--a zinc finger transcription factor, anti-sense plasmid on the invasion of ovary carcinoma cell lines in vitro.
Methods: Four human ovarian carcinoma cell lines ES-2, OVCAR3, HO8910, HO8910PM were analyzed for the expression of Snail and E-cadherin mRNA by RT-PCR. Then anti-Snail plasmid transfection was introduced into HO8910 cells using lipofectin 2000 reagent to investigate the inverse correlation between E-cadherin and Snail expression, and the inhibitory effects of anti-sense Snail were also detected by Transwell motility assay and Matrigel invasion assay.
Results: The results demonstrated the presence of Snail mRNA in ES-2, HO8910, HO8910PM detected by RT-PCR. By contrast, E-cadherin mRNA was only detected in OVCAR3 by RT-PCR. The inverse relationships were further observed by transient transfection of anti-sense Snail into HO8910 cells, which showed the down-regulated expression of Snail and the re-expression of E-cadherin. The Snail mRNA levels were 0.897 +/- 0.005, 0.865 +/- 0.010, 0.338 +/- 0.014 after 0, 24, 48 h of transfection and that of E-cadherin were 0, 0.130 +/- 0.001, 0.217 +/- 0.005 respectively, the difference was significant between different time points (P < 0.05). Besides the reverse correlation of E-cadherin and Snail, we also found that the mRNA level of fibronectin, a mensenchymal marker, was apparently down-regulated after stable transfection of antisense Snail compared to the control cells [0.191 +/- 0.010 vs 0.582 +/- 0.010 (P < 0.01)]. The motility activity and the invasion activity of anti-sense Snail transfectant cells were significantly decreased compared with those of control cells [48 +/- 2 vs 396 +/- 29 (P < 0.01); 35 +/- 10 vs 219 +/- 72 (P < 0.05) respectively].
Conclusions: The data indicate a reverse correlation of E-cadherin and Snail expression exists in ovary carcinoma cells. Snail is possibly a potential target for cancer gene therapy blocking invasion and metastasis in human ovary carcinoma.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
UBE2J1 is identified as a novel plasma cell-related gene involved in the prognosis of high-grade serous ovarian cancer.
J Transl Med
January 2025
Department of Gynecology, The Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Shijiazhuang, 050000, Hebei, China.
Background: Immune cells within tumor tissues play important roles in remodeling the tumor microenvironment, thus affecting tumor progression and the therapeutic response. The current study was designed to identify key markers of plasma cells and explore their role in high-grade serous ovarian cancer (HGSOC).
Methods: We utilized single-cell sequencing data from the Gene Expression Omnibus (GEO) database to identify key immune cell types within HGSOC tissues and to extract related markers via the Seurat package.
Intervention of a Communication Between PI3K/Akt and β-Catenin by (-)-Epigallocatechin-3-Gallate Suppresses TGF-β1-Promoted Epithelial-Mesenchymal Transition and Invasive Phenotype of NSCLC Cells.
Environ Toxicol
January 2025
Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.
The epithelial-mesenchymal transition (EMT) assists in the acquisition of invasiveness, relapse, and resistance in non-small cell lung cancer (NSCLC) and can be caused by the signaling of transforming growth factor-β1 (TGF-β1) through Smad-mediated or Smad-independent pathways. (-)-Epigallocatechin-3-gallate (EGCG), a multifunctional cancer-preventing bioconstituent found in tea polyphenols, has been shown to repress TGF-β1-triggered EMT in the human NSCLC A549 cell line by inhibiting the activation of Smad2 and Erk1/2 or reducing the acetylation of Smad2 and Smad3. However, its impact on the Smad-independent pathway remains unclear.
View Article and Find Full Text PDFEpithelial mesenchymal transition and cancer stem cell markers in oral epithelial dysplasia and oral squamous cell carcinoma.
Pol J Pathol
January 2025
Department of Biology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
The role of cancer stem cells (CSC) in oral cancer is widely accepted. Yet, the existence of CSC in dysplastic tissue and the molecular pathways of progression from dysplasia to malignancy remain to be explored. Our retrospective study aimed to analyze the presence of CSC in oral epithelial dysplasia and oral squamous cell carcinoma (OSCC) concerning two epithelial-mesenchymal transition markers: Snail and E-cadherin.
View Article and Find Full Text PDFNKD2 as a Mediator of IFIX Antioncogene-Induced Wnt Signalling and Epithelial-Mesenchymal Transition in Human OSCC.
J Cell Mol Med
January 2025
Department of Ophthalmology, the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, P. R. China.
The activation of the human interferon-inducible protein X (IFIX) isoform is associated with maintaining a stable cytoskeleton and inhibiting epithelial-mesenchymal transition (EMT). However, the mechanisms and pathways underlying IFIX-mediated oncogenesis are not well understood. In this study, we investigated the effects of IFIX overexpression and knockdown in CAL-27 and SCC-25 oral squamous cell carcinoma (OSCC) cells.
View Article and Find Full Text PDFSophaline B inhibits non-small cell lung cancer by activating NLRP3/caspase-1/GSDMD-dependent pyroptosis and PI3K/AKT/mTOR-mediated autophagy.
Nat Prod Res
January 2025
Institute of Biopharmaceutical and Health Engineering, State Key Laboratory of Chemical Oncogenomics, Shenzhen Key Laboratory of Gene and Antibody Therapy, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, China.
Sophaline B (SPB), extracted from the seeds of L., is a natural bioactive compound that effectively exerts antiviral activities against the hepatitis B virus. This is the first study to demonstrate that SPB exerts anti-tumor effects on NSCLC by inducing pyroptosis and autophagy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!