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Development of a novel protein microarray method for serotyping Salmonella enterica strains. | LitMetric

Development of a novel protein microarray method for serotyping Salmonella enterica strains.

J Clin Microbiol

Laboratory Services Division, University of Guelph, P.O. Box 3650, 95 Stone Road West, Guelph, Ontario, Canada N1H 8J7.

Published: July 2005

AI Article Synopsis

  • An antibody microarray assay was created for Salmonella serotyping using the Kauffmann-White classification.
  • The array featured a model design with 35 antibodies to identify 20 common Salmonella serovars and was tested on 117 target and 73 non-target strains.
  • The assay successfully identified 86 target strains completely, partially identified 30 target strains, and effectively excluded all 73 non-target strains.

Article Abstract

An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allowed complete serovar identification of 86 target strains and partial identification of 30 target strains and allowed exclusion of the 73 nontarget strains from the target serovars.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169117PMC
http://dx.doi.org/10.1128/JCM.43.7.3427-3430.2005DOI Listing

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