[Study on the expression of IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis].

Purpose: To investigate the cell type which express IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis.

Methods: 12 patients with chronic periodontitis who were undergoing flap operation were randomly chosen as periodontitis group;10 patients undergoing gingivectomy were chosen as gingivitis group;6 patients undergoing extraction of impacted teeth were chosen as control group. Assays of in situ hybridization and immunohistochemistry were used to observe the expression of IL-10 mRNA and the protein in gingival tissues of each group respectively . Wilcoxon test was used to compare the expression of IL-10 of each group at two levels; differences between the two groups were analyzed by one-way ANOVA test.

Results: IL-10 mRNA and the protein were all expressed in gingival tissues of periodontium,they were found in lymphocytes, fibroblasts and macrophages. There were no significant differences between two levels of IL-10 expression of periodontitis group and control group (P>0.05) gingivitis group,P<0.05). The IL-10 expression of periodontitis group was significantly higher than gingivitis group and control group (P<0.01); The IL-10 mRNA expression of periodontitis group was significantly higher than control group (P<0.01),but there was no significant difference between the IL-10 mRNA expression of periodontitis group and gingivitis group (P>0.05).

Conclusion: There is a local secretion mechanism of IL-10 in the periodontal tissues.