[Production and releasing sites of superoxide anion in phytopathogenic bacteria].

Many phytopathogenic bacteria were founded to release the superoxide anion (O2-) by themselves from different sites and the production of O2- was related to the pathogenicity of strains. The presences of O2- in cytomembrane, cell wall and filtrate were confirmed by chemical method and electron spin resonance (ESR). The results show that filtrate may be the primary site of O2- production due to its higher O2- generating activity either in the presence or the absence of DDC. Additional lines of evidence further suggested a regulatory mechanism of O2- production in phytopathogenic bacteria.