AI Article Synopsis
- The 042BM noeA gene was amplified using PCR and showed 99% identity with S. meliloti 1021, and 97% similarity in the NoeA protein.
- The NoeA protein demonstrated a 32% similarity with a methyltransferase from Mesorhizobium sp. BNC1, and a 41% similarity with a region of E. coli (PrmA) methyltransferases.
- The noeA deletion mutant 042BMA-Km displayed varying impacts on nodule count and plant weight across different alfalfa cultivars, indicating its role in cultivar-specific nodulation.
Article Abstract
042BM noeA was obtained by PCR. It is identical to that of S. meliloti 1021 at 99% level, and similarity of their NoeA is 97%. In addition, it was found that this protein shares significant homology with the SAM-dependent methyltransferase of Mesorhizobium sp. BNC1 (32% similarity), and the similarity of its 303-362 region to the 160- 220 region of Ll11 methyltransferases of E. coli (PrmA) is 41%. Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu. However, this mutant has no significant change in ability to nodulate cultivars of Huanghou and Zahua. Hence, noeA is involved in alfalfa cultivar-specific nodulation.
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[Cloning, deletion and functional analysis of noeA from Sinorhizobium meliloti 042BM].
Wei Sheng Wu Xue Bao
April 2005
Key Laboratory for Microbial Resources and Application of Agriculture Ministry, Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Article Synopsis
- The 042BM noeA gene was amplified using PCR and showed 99% identity with S. meliloti 1021, and 97% similarity in the NoeA protein.
- The NoeA protein demonstrated a 32% similarity with a methyltransferase from Mesorhizobium sp. BNC1, and a 41% similarity with a region of E. coli (PrmA) methyltransferases.
- The noeA deletion mutant 042BMA-Km displayed varying impacts on nodule count and plant weight across different alfalfa cultivars, indicating its role in cultivar-specific nodulation.
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