AI Article Synopsis
- The study compares the conformational flexibility of two proteins: ferric rat liver outer mitochondrial membrane cytochrome b5 (rOM b5) and ferric bovine liver microsomal cytochrome b5 (bMc b5) using NMR spectroscopy and hydrogen-deuterium exchange (HDX).
- rOM b5 exhibits less conformational flexibility than bMc b5, which may contribute to a higher kinetic barrier for hemin release in rOM b5, affecting its functional properties.
- The restricted mobility in rOM b5 is linked to tighter hydrophobic packing in specific helices (alpha4 and alpha5), likely due to two conserved side chain motifs in outer membrane cytochrome b5 proteins.
Article Abstract
Native-state hydrogen-deuterium exchange (HDX) monitored by NMR spectroscopy has been used to compare conformational plasticity in ferric rat liver outer mitochondrial membrane cytochrome b5 (rOM b5) and ferric bovine liver microsomal cytochrome b5 (bMc b5). Analysis of the data indicated that rOM b5 is the less conformationally flexible protein on the time scale probed by the HDX experiments. The data also suggest a likely contributor to the much higher kinetic barrier for the release of hemin from OM b5s in comparison to Mc b5s, a characteristic that may be to a large extent the source of their divergent functional properties. Specifically, the data indicate that conformational mobility within helices alpha4 and alpha5, which flank the loop harboring axial ligand His63, is considerably more restricted in rOM b5 than in bMc b5. The lower conformational flexibility of alpha4 and alpha5 in rOM b5 can reasonably be attributed to more extensive hydrophobic packing in that region of the protein, arising from two conserved side chain packing motifs in OM cytochrome b5s [Altuve, A., Wang, L., Benson, D. R., and Rivera, M. (2004) Biochem. Biophys. Res. Commun. 314, 602-609].
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| http://dx.doi.org/10.1021/bi050564l | DOI Listing |
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