A study was conducted to determine the efficiency and effectiveness of several techniques for fiber post removal. Four groups of 20 mandibular premolars were endodontically treated and obturated. Post spaces were prepared for the following post systems: ParaPost XH, ParaPost Fiber White, Luscent Anchors, and Aestheti-Plus. After cementation, 10 posts of each group were removed with their corresponding manufacturer's removal kit and the other 10 removed with diamond burs and ultrasonics. Removal times were recorded and the teeth were sectioned vertically and microscopically analyzed for removal effectiveness based on a 0 to 5 point scale. Removal kits removed Luscent Anchors the fastest (mean = 3.9 min) and most effectively (mean = 2.6), while Aestheti-Plus posts were removed the slowest (mean = 7.3 min) and least effectively (mean = 3.4). Diamonds and ultrasonics required an average of 10 additional minutes for each fiber post system removal, yet removal effectiveness improved half a point. The results suggest recommended removal kits were significantly more efficient, while diamonds and ultrasonics were more effective. Removal kits could be enhanced with subsequent ultrasonic instrumentation to remove remaining fibers and cement.
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http://dx.doi.org/10.1097/01.don.0000167397.60943.6e | DOI Listing |
Appl Environ Microbiol
January 2025
Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA.
As a diverse and complex food matrix, the animal food microbiota and repertoire of antimicrobial resistance (AMR) genes remain to be better understood. In this study, 16S rRNA gene amplicon sequencing and shotgun metagenomics were applied to three types of animal food samples (cattle feed, dry dog food, and poultry feed). ZymoBIOMICS mock microbial community was used for workflow optimization including DNA extraction kits and bead-beating conditions.
View Article and Find Full Text PDFJ Environ Manage
January 2025
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing, 100012, PR China; Research Center of Environmental Pollution Control Engineering Technology, Chinese Research Academy of Environmental Sciences, Beijing, 100012, PR China. Electronic address:
Biological acidification, efficient and low-cost biotechnology, is crucial in treating pharmaceutical, pesticide water, and petrochemical wastewater. Nitrobenzene is a typical organic pollutant in petrochemical wastewater with high toxicity and long persistence. However, its effect on hydrolysis acidification is yet to be fully elucidated.
View Article and Find Full Text PDFMicrob Pathog
February 2025
Hospital Israelita Albert Einstein, Av. Albert Einstein 627, Morumbi, São Paulo, 05651-901, Brazil; Departamento de Obstetricia e Ginecologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
Endometriosis is a chronic gynecological disease that affects 10 % of reproductive-aged women and characterized by the presence of endometrial tissue outside the uterus. The disease is linked to a pro-inflammatory environment in the peritoneal fluid of patients, with high levels of cytokines, growth factors, and reactive oxygen species. Changes in the peritoneal fluid, such as altered immune cells and cytokines, can be linked to the immune balance in endometriosis.
View Article and Find Full Text PDFObjective: To describe indications for and demonstrate the technique to perform a transtracheal aspirate in horses. Transtracheal aspirate is indicated to collect samples for cytology and bacteriologic culture in cases of suspected pneumonia or other lower respiratory tract disease.
Animals: 1 healthy university-owned horse was used for demonstration purposes.
Front Genet
December 2024
Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, BC, Canada.
The advent of long-read (LR) sequencing technologies has provided a direct opportunity to determine the structure of transcripts with potential for end-to-end sequencing of full-length RNAs. LR methods that have been described to date include commercial offerings from Oxford Nanopore Technologies (ONT) and Pacific Biosciences. These kits are based on selection of polyadenylated (polyA+) RNAs and/or oligo-dT priming of reverse transcription.
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