Rat fetal neural stem cells (rFNSCs) was separated from embryo about 14.5-16.5 days, and cultured in DMEM/F12 media with additives and epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Effects of lipid on growth and proliferation of rFNSCs was examined by counting the number of neurospheres and incorporation of 3H. The data show that chemical defined lipid improved rFNSCs' growth and cell division. Lipid will be another neural stem cell's culture media's additive.
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