Arf-like proteins (Arl) share certain characteristic features with the Arf subfamily of Ras superfamily proteins, but their function is unknown. Here, we show by a variety of spectroscopic techniques that Arl2, unlike most other Ras-related proteins, has micromolar rather than picomolar affinity for nucleotides. As a consequence of low affinity, nucleotide dissociation rates are rather fast, arguing that it is not regulated by guanine nucleotide exchange factors. Arl2 is isolated as prey in a yeast double hybrid screen using phosphodiesterase 6delta (PDEdelta) as bait. This interaction is dependent on GTP, and the binding of PDEdelta substantially stabilizes GTP binding, increasing affinity and decreasing dissociation rates by a similar factor. Among all Arl proteins tested, PDEdelta only interacted with the closely related proteins Arl2 and Arl3, strongly suggesting that Arl2/3 are specific regulators of PDEdelta.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jmb.2005.05.036 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dually Fluorinated Unimolecular Micelles for Stable Oxygen-Carrying and Enhanced Photosensitive Efficiency to Boost Photodynamic Therapy against Hypoxic Tumors.
Acta Biomater
January 2025
State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials; Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application; Suzhou key Laboratory of Macromolecular Deign and Precision Synthesis; College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China. Electronic address:
Tumor hypoxia is one of key challenges in deep tumor photodynamic therapy (PDT), and how to fix this issue is attracting ongoing concerns worldwide. This work demonstrates dually fluorinated unimolecular micelles with desirable and stable oxygen-carrying capacity, high cellular penetration, and integrative type I & II PDT for deep hypoxic tumors. Dually fluorinated star copolymers with fluorinated phthalocyanines as the core are prepared through photoinitiated electron/energy transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization under irradiation with NIR LED light at room temperature, followed by assembly into unimolecular micelles.
View Article and Find Full Text PDFEvidence for low bioavailability of dietary nanoparticulate cerium in a freshwater food chain.
Aquat Toxicol
December 2024
ANSTO, Nuclear Science and Technology Division, Lucas Heights, NSW 2234, Australia.
Radioactive Ce in ionic (I-Ce), nano (N-Ce, 11 ± 9 nm mean primary particle size ± standard deviation) and micron-sized (M-Ce, 530 ± 440 µm) forms associated with natural and artificial diets in natural river water and synthetic freshwater were used to measure the real-time biokinetics of dietary Ce assimilation in a freshwater food chain. The model food chain consisted of microalgae (Raphidocelis subcapitata), snails (Potamopyrgus antipodarum) and prawns (Macrobrachium australiense). Pulse-chase experiments showed that 91-100 % of all forms of cerium associated with all diets and water types were eliminated from the digestive system of the snail and prawn within 24 h, with no detectable cerium assimilation.
View Article and Find Full Text PDFContinuous theta-burst stimulation demonstrates language-network-specific causal effects on syntactic processing.
Neuroimage
January 2025
Max Planck Partner Group, School of International Chinese Language Education, Beijing Normal University, Beijing, China; Department of Neuropsychology, Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany. Electronic address:
Hierarchical syntactic structure processing is proposed to be at the core of the human language faculty. Syntactic processing is supported by the left fronto-temporal language network, including a core area in the inferior frontal gyrus as well as its interaction with the posterior temporal lobe (i.e.
View Article and Find Full Text PDFUltrasensitive dual-mode biosensor for photoelectrochemical and differential pulse voltammetry detection of thrombin based on DNA self-assembly.
Biosens Bioelectron
January 2025
Hebei Key Laboratory of Nano-Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China. Electronic address:
Abnormal levels of thrombin may be associated with various diseases, such as thrombosis and hemorrhagic diseases, making precise detection of thrombin particularly important. Dual signal detection is a method that enhances detection sensitivity and specificity by simultaneously utilizing two different signals. Its primary advantages include improving detection accuracy and reducing false positive rates, making it particularly suitable for clinical analysis and diagnostics.
View Article and Find Full Text PDFAmorphous/Crystalline ZrO with Oxygen Vacancies Anchored Nano-Ru Enhance Reverse Hydrogen Spillover in Alkaline Hydrogen Evolution.
Small
January 2025
State Key Laboratory of Bio-Fibers and Eco-Textiles, College of Materials Science and Engineering, College of Environmental Science and Engineering, Qingdao University, Qingdao, 266071, P. R. China.
Article Synopsis
- A hydrogen spillover-based binary (HSBB) system is gaining attention for improving alkaline hydrogen evolution reactions (HER) by enhancing the spillover process.
- The innovation involves anchoring nano-Ru onto oxygen vacancy-rich amorphous/crystal ZrO, which helps water molecules break down into protons that generate hydrogen.
- The unique structure increases hydrogen adsorption/desorption rates, reduces work function for better electron transfer, and results in impressive performance metrics, outperforming commercial Pt/C catalysts.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!