Purpose: To study the rate of curing, three composites, Heliomolar (Vivadent), Z100 (3M), and Renew (Bisco) were investigated
Methods: Volumetric shrinkage was measured at 25 degrees C using the Acuvol with an RG610 red filter. The dynamic measurements were made in the single view mode.
Results: Detailed kinetic studies for Renew determined the effect of varying the light intensity (100mW - 500mW) and irradiation time (3 seconds - 30 seconds) on the rate of curing. A measurement of the gel time of composites and a kinetic constant is reported. ANOVA followed by a Fisher's LSD test and a Kruskall-Wallis test were used for analysis of the data. The gel times follow the order of Heliomolar > Renew > Z100 at 20 seconds and 500 mW. Irradiation time had no significant effect on the gel time of Renew. Light intensity had a significant effect on the gel time of Renew.
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Anal Chim Acta
February 2025
Food Laboratory of Zhongyuan, Luohe, 462000, Henan Province, PR China.
Background: Edible oils are susceptible to contamination with polycyclic aromatic hydrocarbons (PAHs) throughout production, storage, and transportation processes due to their lipophilic nature. The necessity of quantifying PAHs present in complex oil matrices at trace levels, which bind strongly to impurities in oil matrices, poses a major challenge to the accurate quantification of these contaminants. Therefore, the development of straightforward and effective methods for the separation and enrichment of PAHs in oil samples prior to instrumental analysis is paramount to guaranteeing food safety.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing, 100081, China. Electronic address:
Background: The metal organic cages (MOCs) are an emerging type of porous material that has attracted considerable research interest due to their unique properties, including good stability and well-defined intrinsic cavities. The chiral MOCs with porous structures have broad application prospects in enantiomeric recognition and separation. However, there are almost no relevant reports on chiral MOCs as chiral stationary phases (CSPs) for enantioseparation by high-performance liquid chromatography (HPLC).
View Article and Find Full Text PDFActa Biomater
January 2025
Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, TX, 78712, USA. Electronic address:
The design of biomaterials that can reconfigure on-demand in response to external stimuli is an emerging area in materials research. However, achieving reversible assembly of protein-based biomaterials by light input remains a major challenge. Here, we present the engineering of a new protein material that is capable of switching between liquid and solid state reversibly, controlled by lights of different wavelengths.
View Article and Find Full Text PDFJ Mol Biol
January 2025
Department of Chemistry and Biochemistry, California State University, San Bernardino, 5500 University Pkwy, San Bernardino, CA 92407, USA. Electronic address:
So far, site-directed alkylation (SDA) studies on transporters in the Major Facilitator Superfamily (MFS) are mostly performed at conditions different from the native cellular environment. In this study, using GFP-based site-directed PEGylation, ligand-induced conformational changes in the lactose permease of Escherichia coli (LacY), were examined in vivo for the first time. Accessibility/reactivity of single-Cys replacements in a Cys-less LacY-eGFP fusion background was tested using methoxy polyethylene glycol-maleimide-5K (mPEG-Mal-5K) in the absence or presence of a ligand, and the band-shift of the fusion upon PEGylation was detected by in-gel fluorescence.
View Article and Find Full Text PDFPhytomedicine
December 2024
Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100073, China. Electronic address:
Background: Adherence of Helicobacter pylori to the surface of the gastric mucosa is the initial and crucial step for its survival and colonization in the harsh conditions of the stomach. We had previously demonstrated that daphnetin has anti-adhesion effect.
Purpose: This study aims to explore the mechanisms of daphnetin to reduce H.
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