A raw starch utilizing microbe was isolated from mud in a milling factory. The 16S ribosomal DNA (rDNA) sequencing and morphological properties of the strain indicated that it belongs to the genus Streptomyces. A strongly raw starch digesting amylase was purified from the culture supernatant of the strain by chromatographic procedures. The specific activity of the enzyme was 11.7 U/mg, molecular mass 47 kDa, optimum pH 6.0, and optimum temperature 50 to 60 degrees C. The enzyme showed sufficient activity even at 70 degrees C. It was activated by calcium, cobaltous, and magnesium ions, and inhibited by copper, nickel, zinc, and ferrous ions. It formed maltose mainly from raw and gelatinized starch, and glycogen. No products were formed from glucose, maltose, maltotriose, pullulan, or cyclodextrins (CDs). The enzyme digested raw wheat, rice, and waxy rice starch rapidly, and raw corn, waxy corn, sweet potato, tapioca, and potato starch normally.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1271/bbb.69.1073 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-affinity uric acid clearance based on motile β-CD/F-127 polyrotaxane microspheres for enhanced diabetic wound repair.
Carbohydr Polym
March 2025
State Key Laboratory of Bioactive Molecules and Druggability Assessment, Institute of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address:
Hyperuricemia-related diabetic wounds are notoriously difficult to treat due to elevated uric acid (UA) levels, excessive reactive oxygen species (ROS), and chronic inflammation. Current therapies often fail to address these underlying causes, underscoring the need for innovative approaches that not only clear UA but also mitigate inflammation and promote tissue regeneration. In this study, we developed a polyrotaxane-based microsphere (HPR MS) system conjugated with 4,5-diamino-2-thiouracil (DT) to achieve high-affinity UA clearance without increasing cytotoxicity.
View Article and Find Full Text PDFBiodegradation Assessment of Bioplastic Carrier Bags Under Industrial-Scale Composting Conditions.
Polymers (Basel)
December 2024
Institute of Environmental Sciences, Hungarian University of Agriculture and Life Sciences, Páter Károly u. 1, H-2100 Gödöllő, Hungary.
In recent years, the environmental impacts of plastic production and consumption have become increasingly significant, particularly due to their petroleum-based origins and the substantial waste management challenges they pose. Currently, global plastic waste production has reached 413.8 million metric tons across 192 countries, contributing notably to greenhouse gas emissions.
View Article and Find Full Text PDFProduction of Starch-Based Flexible Food Packaging in Developing Countries: Analysis of the Processes, Challenges, and Requirements.
Foods
December 2024
Food Packaging and Shelf Life Laboratory, Instituto de Ciencia y Tecnología de Alimentos, Universidad Nacional de Colombia, Carrera 30 Número 45-03, Edificio 500A, Bogotá 111321, Colombia.
Biodegradable packaging offers an affordable and sustainable solution to global pollution, particularly in developing countries with limited recycling infrastructure. Starch is well suited to develop biodegradable packages for foods due to its wide availability and simple, low-tech production process. Although the development of starch-based packaging is well documented, most studies focus on the laboratory stages of formulation and plasticization, leaving gaps in understanding key phases such as raw material conditioning, industrial-scale molding, post-production processes, and storage.
View Article and Find Full Text PDFImpact of Cooking on Starch Digestibility in Foxtail Millets.
Foods
December 2024
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
While the digestibility of millet starch has been studied considerably, the effects of cooking on starch digestibility in millet remain insufficiently understood. This study investigated the effects of cooking on in vitro enzymatic starch digestion in eight cooked millet flour cultivars by seeking its correlations with the changes in composition (moisture, total starch, protein, lipids, total dietary fiber, and phenolics), structure, and physicochemical properties. Compared to raw flours, cooked flours had a similar content of total starch and protein, a lower content of moisture, lipids, and total phenolic content, and a higher content of total dietary fiber.
View Article and Find Full Text PDFInsight into the Use of Brewers' Spent Grain as a Low-Carbon Aggregate in Building Materials.
Biomimetics (Basel)
December 2024
Institut de Recherche ESTP, 94230 Cachan, France.
This study investigates the use of Brewers' Spent Grains (BSGs) as a sustainable biocomposite building materials, using cornstarch as a biopolymer binder. BSG aggregates are compared with hemp shives, a conventional aggregate known for its thermal properties. Starch is employed as a natural binder in three different formulations to further reduce the carbon footprint of the building material.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!