AI Article Synopsis
- Spiropyran dyes were chemically linked to CdSe/ZnS nanocrystals using thiol linkers, allowing for UV-triggered photoisomerization.
- The isomerization process significantly reduced the fluorescence of the nanoparticles, which could be restored by exposing them to visible light.
- The efficiency of fluorescence quenching depended on how well the emission spectrum of the nanoparticles overlapped with the absorption bands of the merocyanine form of the dye, indicating a FRET (Förster resonance energy transfer) mechanism at play, with complete quenching needing at least 80 dye molecules per nanoparticle.
Article Abstract
Spiropyran dyes were attached to fluorescent core-shell CdSe/ZnS nanocrystals via thiol-containing linkers. Photoisomerization of the dye to its merocyanine form by UV irradiation caused a dramatic loss in the intrinsic nanoparticle fluorescence, which was regained upon reversing the isomerization with visible light. The fluorescence quenching efficiency increased with increasing spectral overlap of fluorescence emission and merocyanine adsorption bands, consistent with FRET as the quenching mechanism. Typically, complete quenching required at least 80 bound dye molecules per particle.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435833 | PMC |
| http://dx.doi.org/10.1021/ja0423421 | DOI Listing |
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