Methanosarcina barkeri DSM-804, a methanogenic bacterium, when exposed to microwave radiation of frequencies ranging from 13.5 to 36.5 GHz, showed faster growth in comparison to the unirradiated bacterial culture. Methane concentration in the biogas generated from the irradiated culture was higher than that from unirradiated one, which was to 76.3% on the 15th day of incubation at a microwave radiation frequency of 31.5 GHz, 10 dbm power when irradiated for 2 h. Microscopic study of pure culture revealed that the cells of M. barkeri were more in number and their cell diameter was enlarged by 20%. Inoculation of the culture in a biogas digester containing a combination of jute waste and vegetable market waste as substrate increased the efficacy of biomethanation and reduced its lag phase significantly.
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http://dx.doi.org/10.1016/j.biortech.2005.04.032 | DOI Listing |
J Basic Microbiol
December 2024
Department of Bioinformatics, Industrial Systems Biology Lab, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India.
Sci Total Environ
December 2024
College of Urban Construction, Nanjing Tech University, Nanjing 211816, PR China.
Existing researches involving accelerated interspecies electron transfer (IET) by solid redox mediators focus mainly on the conductive nature of these materials. Although non-conductive solid redox mediator-humin has been reported to promote methanogenic performance of anaerobic granular sludge, likely through accelerating IET of microorganisms, this phenomenon has not been validly proven. In this study, a wetland sediment sourced HM (HM) was added into a co-culture of a syntrophic bacteria Shewanella oneidensis MR-1 and an archaeal Methanosarcina barkeri with ethanol as sole electron donor to examine whether HM can accelerate the IET between these two species.
View Article and Find Full Text PDFWater Res
January 2025
Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China. Electronic address:
Effective treatment of high-concentration brewery wastewater through anaerobic digestion (AD) has always been a challenging issue. Enhancing direct interspecies electron transfer (DIET) was demonstrated to increase methane production during AD under high organic loading rate (OLR). Herein, the feasibility of enhancing DIET with the addition of riboflavin-loaded granular activated carbon (RF-GAC) as well as co-addition with Methanosarcina barkeri (Rf-GAC+M.
View Article and Find Full Text PDFEnviron Sci Technol
November 2024
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Environ Sci Technol
October 2024
Key Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Humic substances are organic substances prevalent in various natural environments, such as wetlands, which are globally important sources of methane (CH) emissions. Extracellular electron transfer (EET)-mediated anaerobic oxidation of methane (AOM)-coupled with humic substances reduction plays an important role in the reduction of methane emissions from wetlands, where magnetite is prevalent. However, little is known about the magnetite-mediated EET mechanisms in AOM-coupled humic substances reduction.
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