AI Article Synopsis
- Rhizobial bacteria form a symbiotic relationship with legumes, triggering various responses in roots via a signaling molecule called Nod factor.
- The study identifies NSP2, a GRAS protein from Medicago truncatula, as crucial for the signaling process initiated by Nod factor, acting downstream of calcium spikes.
- NSP2-GFP localizes to the endoplasmic reticulum/nuclear envelope and moves to the nucleus after exposure to Nod factor, suggesting its role in mediating calcium signals and regulating gene expression related to Nod-factor responses.
Article Abstract
Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes a GRAS protein essential for Nod-factor signaling. NSP2 functions downstream of Nod-factor-induced calcium spiking and a calcium/calmodulin-dependent protein kinase. We show that NSP2-GFP expressed from a constitutive promoter is localized to the endoplasmic reticulum/nuclear envelope and relocalizes to the nucleus after Nod-factor elicitation. This work provides evidence that a GRAS protein transduces calcium signals in plants and provides a possible regulator of Nod-factor-inducible gene expression.
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| http://dx.doi.org/10.1126/science.1110951 | DOI Listing |
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