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On-resin multicomponent protocols for biopolymer assembly and derivatization.
Nat Protoc
February 2021
Laboratory for Organic & Microwave-Assisted Chemistry (LOMAC), Department of Chemistry, University of Leuven (KU Leuven), Leuven, Belgium.
Solid-phase synthesis represents the methodological showcase for technological advances such as split-and-pool combinatorial chemistry and the automated synthesis of peptides, nucleic acids and polysaccharides. These strategies involve iterative coupling cycles that do not generate functional diversity besides that incorporated by the amino acids, nucleosides and monosaccharide building blocks. In sharp contrast, multicomponent reactions (MCRs) are traditionally used to generate both skeletal and appendage diversity in short, batchwise procedures.
View Article and Find Full Text PDFAdvanced immunocapture of milk-borne Salmonella by microfluidic magnetically stabilized fluidized bed.
Electrophoresis
February 2018
Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
The success of microfluidic immunocapture based on magnetic beads depends primarily on a sophisticated microscale separation system and on the quality of the magnetic immunosorbent. A microfluidic chip containing a magnetically stabilized fluidized bed (μMSFB), developed for the capture and on-chip amplification of bacteria, was recently described by Pereiro et al..
View Article and Find Full Text PDFCatalyst recycling via specific non-covalent adsorption on modified silicas.
Dalton Trans
March 2013
InCatT B.V. Science Park 904, 1098 XH Amsterdam, The Netherlands.
Article Synopsis
- The article details a new method for recycling a hydroformylation catalyst using selective adsorption to custom-designed supports after the reaction.
- The catalyst separation relies on non-covalent interactions between a ligand and the support, allowing the active catalyst to be released for reuse by changing the solvent.
- A specific rhodium pre-catalyst shows high stability and efficiency, achieving a turnover number of 170,000, with successful recycling demonstrated through multiple runs using end-capped silica-alumina supports.
Fluorescence biosensing micropatterned surfaces based on immobilized human acetylcholinesterase.
Anal Bioanal Chem
January 2013
Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.
Human acetylcholinesterase (AChE) is a widely studied target enzyme in drug discovery for Alzheimer's disease (AD). In this paper we report evaluation of the optimum structure and chemistry of the supporting material for a new AChE-based fluorescence sensing surface. To achieve this objective, multilayered silicon wafers with spatially controlled geometry and chemical diversity were fabricated.
View Article and Find Full Text PDFAffinity adsorption of lysozyme on a macroligand prepared with Cibacron Blue 3GA attached to yeast cells.
J Chromatogr B Analyt Technol Biomed Life Sci
September 2011
Laboratory of Molecular Biology and Biotechnology, Department of Biochemistry, San Luis University, Ejército de los Andes 950, D5700HHW, San Luis, Argentina.
The objective of this study was the development of affinity adsorbent particles with the appropriate characteristics to be applied in protein purification using the affinity ultrafiltration method. To prepare affinity macroligands Cibacron Blue 3GA, as a ligand molecule, was immobilized by covalent bonding onto yeast cell walls, the support material or matrix. The maximum attachment of the ligand to the matrix was 212 μmol/g (ligand dry weight/yeast dry weight).
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