A quartz crystal microbalance (QCM) DNA sensor, based on the nanoparticle amplification method, was developed for detection of Escherichia coli O157:H7. A thiolated single-stranded DNA (ssDNA) probe specific to E. coli O157:H7 eaeA gene was immobilized onto the QCM sensor surface through self-assembly. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA, and resulted in the mass change and therefore frequency change of the QCM. Streptavidin conjugated Fe(3)O(4) nanoparticles (average diameter=145 nm) were used as "mass enhancers" to amplify the frequency change. Synthesized biotinylated oligonucleotides as well as E. coli O157:H7 eaeA gene fragments (151 bases) amplified using asymmetric PCR with biotin labeled primers were tested. As low as 10(-12)M synthesized oligonucleotides and 2.67 x 10(2) colony forming unit (CFU)/ml E. coli O157:H7 cells can be detected by the sensor. Linear correlation between frequency change and logarithmic number of bacterial cell concentration was found for E. coli O157:H7 from 2.67 x 10(2) to 2.67 x 10(6)CFU/ml.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2005.04.021 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of active composite coating based on nanochitosan-whey protein isolate on the microbial safety of chilled rainbow trout fillets packed with oxygen absorber.
Int J Biol Macromol
October 2024
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Article Synopsis
- This study evaluated how a coating made from nanochitosan and whey protein isolate, combined with summer savory essential oil and oxygen absorber packaging, affects the growth of specific bacteria on rainbow trout fillets stored in the fridge.
- Results showed that while Listeria monocytogenes initially decreased in all treatment groups, it eventually increased, indicating limited effectiveness of the treatments against this bacterium.
- In contrast, the combined treatment with the coating, essential oil, and oxygen absorber was most effective in slowing the growth of E. coli and reducing Pseudomonas aeruginosa levels compared to other methods.
Detection of O157H7 and in Bovine Carcasses in Two Slaughterhouses in Bio-Bío District, Chile.
Foodborne Pathog Dis
July 2024
Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Concepción, Chile.
Article Synopsis
- * Out of 544 samples analyzed from 272 bovine carcasses, 11 tested positive for O157:H7 (4.0%), and 6 of those had specific pathogenicity genes detected.
- * Additionally, 22 carcasses (8.0%) tested positive for the unspecified pathogen, but none contained the specific genes associated with pathogenicity.
[Evaluation of effect based on different typing methods in ].
Zhonghua Liu Xing Bing Xue Za Zhi
August 2022
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
To evaluate the typing and clinical application effect based on clustered regularly interspaced short palindromic repeats (CRISPRs), serotype, and Multilocus Sequence Typing (MLST). The spacers, serotype and sequence type (ST) were obtained with CRISPRsFinder, SeroTypeFinder and MLST. PCR was used to amplify the CRISPRs, and the spacers were used to predict serotype and ST, then comparing with the serotype and ST.
View Article and Find Full Text PDFRapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe.
RSC Adv
January 2022
College of Artificial Intelligence, Guangdong Mechanical & Electrical Polytechnic Guangzhou 510550 P. R. China +86-20-36552429 +86-20-36552429.
Rapid measurement of waterborne bacterial viability is crucial for ensuring the safety of public health. Herein, we proposed a colorimetric assay for rapid measurement of waterborne bacterial viability based on a difunctional gold nanoprobe (dGNP). This versatile dGNP is composed of bacteria recognizing parts and signal indicating parts, and can generate color signals while recognizing bacterial suspensions of different viabilities.
View Article and Find Full Text PDFInvestigation of phage and molasses interactions for the biocontrol of O157:H7.
Can J Microbiol
September 2021
Department of Food Engineering, Beytepe, Hacettepe University, Ankara, Turkey.
Article Synopsis
- Antibiotic resistance is a major global health issue, prompting researchers to seek alternative treatment methods.
- Molasses shows promising antimicrobial properties that may help combat resistant bacteria by disrupting bacterial cell membranes and proteins.
- The study found that although molasses did not work synergistically with phages, it increased the size of phage plaques and enhanced their activity, suggesting potential benefits of using molasses alongside phage therapy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!