The purpose of this study was to investigate factors affecting mechanical- and cryo-resistance of the rumen ciliates Entodinium caudatum (E.c.), Entodinium furca monolobum (E.f.m.), Entodinium simplex (E.s.), Diplodinium denticulatum (two clones, D.d.01 and D.d.02), Diploplastron affine (D.a.) and Epidinium ecaudatum forma caudatum (E.e.c.) after long-term in vitro cultivation. Following prolonged in vitro cultivation (more than six months), the ciliates were very sensitive to both centrifugation and 5% (v/v) dimethylsulphoxide, with motility decreased to: 39 and 23% for E.c., 66 and 32% for E.f.m., 46 and 27% for D.d. 01, 64 and 41% for D.a., and 44 and 28% for E.e.c., respectively. Thus, cryopreservation was unsuccessful. The effect of supplementing the ciliate growth medium with rumen fluid, glycine-betaine, proline, myo-inositol, linoleic acid, Sel-Plex or insulin, together with the effect of the source of rumen fluid on ciliate resistance to centrifugation, dimethylsulphoxide and freezing was also tested. The omission of rumen fluid from the growth medium resulted in the loss of cryoresistance after one-month cultivation. Supplementing the growth environment with a combination of glycine-betaine, proline, linoleic acid, Sel-Plex, insulin plus improved quality rumen fluid significantly enhanced survival of the ciliates after the freezing-thawing procedure (from 1 to 33% survival in un-supplemented vs. supplemented for E.c., P<0.01; 4-40% E.f.m., P<0.01; 0-17% D.d., P<0.05; 5-7% D.a. and 4-36% E.e.c., P<0.01).
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http://dx.doi.org/10.1016/j.cryobiol.2005.05.001 | DOI Listing |
Introduction: The residual black wolfberry fruit (RBWF) is rich in nutrients and contains a diverse range of active substances, which may offer a viable alternative to antibiotics. This experiment was conducted to investigate the impact of varying levels of RBWF on the growth performance and rumen microorganisms of fattening sheep, and to quantify its economic benefits.
Methods: In this experiment, 40 three-month-old and male Duolang sheep with an average weight of 29.
Int J Biol Macromol
January 2025
State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Centre of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. Electronic address:
This study investigated the effects of rumen-degradable starch (RDS) on lactation performance, gastrointestinal fermentation, and plasma metabolomics in dairy cows. Six mid-lactation cows, fitted with rumen, duodenum, and ileum cannulas, were used in a duplicated 3 × 3 Latin square design with 28-day periods. The cows were fed a low RDS (LRDS; 62.
View Article and Find Full Text PDFAnimals (Basel)
January 2025
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
The present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 ± 0.
View Article and Find Full Text PDFToxins (Basel)
December 2024
Department of Food Safety and Quality, Faculty of Veterinary, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania.
The degradation of zearalenone (ZEN) in the rumen of dairy cows is influenced by rumen pH, which is a key factor affecting this process. The aim of this study was to investigate the variation of ZEN in interaction with other mycotoxins at different ruminal pH environments (physiological (pH 6.5) and acidic (pH 5.
View Article and Find Full Text PDFVet Sci
January 2025
Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Nanning 530001, China.
The current study investigated the in vitro degradability, in vitro gas production, methane (CH) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. Results reveal that crude protein (CP) concentration was the highest at the 220 cm height compared with the other heights.
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