A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionflbqblnk9b9isjr8lk0r9i9f0hfife5m): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

Three-dimensional polarization sensitizes hepatocytes to Fas/CD95 apoptotic signalling. | LitMetric

AI Article Synopsis

  • * Murine hepatocytes cultured in a 3D Matrigel matrix formed organoids featuring organized structures and functional characteristics, like albumin secretion.
  • * Upon stimulation of the hepatotoxic Fas/CD95 death receptor, these organoids showed increased apoptosis, contrasting with monolayer cultures that were protected due to enhanced NF-kappaB signaling.

Article Abstract

Maintenance of epithelial cell shape and polarity determines many vital cell functions, including the appropriate response to external stimuli. Murine hepatocytes cultured in a three-dimensional Matrigel matrix formed highly polarized organoids characterized by specific localization of an ERM (ezrin/radixin/moesin) protein, radixin, at microvillus-lined membrane domains. These apical domains surrounded a lumen and were bordered by tight junctions. The hepatocyte organoids were functional as judged by the high level of albumin secretion and accumulation of bilirubin. Stimulation of the Fas/CD95 death receptor, which is highly hepatotoxic in vivo, was a strong inducer of apoptosis in the polarized organoids. This was in sharp contrast to the monolayer hepatocyte cultures, which were protected from death by exacerbated NF-kappaB signalling following engagement of the death receptors. Thus, hepatocytes in polarized, functional organoids modulate an intracellular signal transduction pathway, allowing the recapitulation of their physiological response to an apoptotic stimulus.

Download full-text PDF

Source
http://dx.doi.org/10.1242/jcs.02403DOI Listing

Publication Analysis

Top Keywords

polarized organoids
8
three-dimensional polarization
4
polarization sensitizes
4
sensitizes hepatocytes
4
hepatocytes fas/cd95
4
fas/cd95 apoptotic
4
apoptotic signalling
4
signalling maintenance
4
maintenance epithelial
4
epithelial cell
4

Similar Publications

Taybi-Linder syndrome (TALS) is a rare autosomal recessive disorder characterized by severe microcephaly with abnormal gyral pattern, severe growth retardation and bone abnormalities. It is caused by pathogenic variants in the RNU4ATAC gene. Its transcript, the small nuclear RNA U4atac, is involved in the excision of ~850 minor introns.

View Article and Find Full Text PDF

H19 promotes polarization and alternative splicing in tumor-associated macrophages, facilitating pancreatic cancer progression.

Cancer Lett

December 2024

Department of General Surgery, Pancreatic Disease Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; Research Institute of Pancreatic Diseases, Shanghai Key Laboratory of Translational Research for Pancreatic Neoplasms, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; State Key Laboratory of Oncogenes and Related Genes, Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. Electronic address:

Tumor-associated macrophages (TAMs) play a crucial physiological role in the pancreatic tumor microenvironment. However, the role of long non-coding RNAs (lncRNAs) in TAMs within pancreatic tumors remains unclear. By lncRNA sequencing between TAMs and resident macrophages from normal tissues in pancreatic cancer, it is found that H19 is highly expressed in TAMs and is correlated with the prognosis and stages of pancreatic cancer.

View Article and Find Full Text PDF

Background: An increasing number of studies have focused on ailanthone (aila) due to its antitumor activity. However, the role of ailanthone in glioblastoma(GBM) has not been investigated before. This study aims to explore the biological function and the underlying mechanism of ailanthone in GBM.

View Article and Find Full Text PDF

Precancerous lesions of gastric cancer (PLGC) are considered critical stages for the prevention and treatment of gastric cancer (GC), with gastric mucosal inflammation being a prerequisite for PLGC. Macrophages, integral to the immune system, typically respond to external stimuli triggering inflammation. Celastrus orbiculatus Thunb.

View Article and Find Full Text PDF
Article Synopsis
  • 3D cell cultures, particularly patient-derived organoids (PDOs), are revolutionizing research on pancreatic functions and diseases, tackling major challenges like pancreatitis.* -
  • The article provides detailed protocols for creating and maintaining pancreatic PDOs, including techniques for polarity switching, and includes a troubleshooting guide.* -
  • By outlining both the processes and cost considerations, the authors encourage a shift from using rodent models to primary human cells, supporting efforts to improve clinical research reliability.*
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!