AI Article Synopsis
- Reactive oxygen species (ROS) can trigger cell death, and researchers studied their impact on mitochondria in transgenic Arabidopsis plants with GFP to visualize changes.
- Leaves were exposed to ROS and related chemicals, and microscopic techniques were used to observe mitochondrial behavior and monitor electrolyte leakage.
- The study found that ROS treatment caused mitochondria to alter their shape, shrink in size, and block cytoplasmic streaming, indicating a link between these changes and cell death under ROS stress.
Article Abstract
Background And Aims: Reactive oxygen species (ROS) are involved in triggering cell death. To visualize mitochondrial behaviour under ROS stress, transgenic arabidopsis plants possessing mitochondrial-targeted GFP (S65T) were studied.
Methods: Arabidopsis leaves were treated with ROS and ROS-inducing chemicals such as hydrogen peroxide, paraquat and menadione. Microscopic observations were carried out using a confocal laser scanning microscope system, and electrolyte leakage was also monitored.
Key Results: After treatment, mitochondria showed morphological changes from a bacillus-like to a round shape. The size of mitochondria treated with H(2)O(2) decreased by half compared with controls. Concurrently, cytoplasmic streaming was blocked and mitochondria eventually swelled. Treatment of leaves with butanedione monoxime, an inhibitor of myosin ATPase, resulted in similar behaviour of mitochondria to that under ROS stress.
Conclusions: The results indicate that morphological changes of mitochondria and cessation of cytoplasmic streaming may interact, and this phenomenon is one of the features of ROS stress-induced cell death.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246881 | PMC |
| http://dx.doi.org/10.1093/aob/mci181 | DOI Listing |
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