AI Article Synopsis
- PTTG is a gene that resembles securin and is important in cell transformation, but its role in normal liver tissues is not well understood, particularly during liver regeneration.
- PTTG expression in rat liver cells increased with hepatocyte growth factor (HGF) and decreased with transforming growth factor-beta1, indicating its involvement in liver cell proliferation.
- The study suggests that PTTG could serve as a new marker for tracking liver cell proliferation during regeneration processes in both laboratory and living subjects.
Article Abstract
Background: Pituitary tumor transforming gene (PTTG), homologous to a mammalian securin, plays a pivotal role in cell transformation, however, its biological function(s) in normal tissues is not fully understood. Because the liver is a regenerative organ, the relevant biological function of PTTG in the liver would be more feasible to understand PTTG. Also, PTTG may be involved in the liver regeneration.
Materials And Methods: Expressions of rat hepatic PTTG messengerRNA (mRNA) and cellular immunoreactivities during the cell proliferative period of the liver regeneration both in vitro and in vivo were tested.
Results: PTTG expression of the rat primary hepatocyte was stimulated by HGF in a dose dependent manner, and was suppressed when hepatocyte proliferation was inhibited by transforming growth factor-beta1. A positive PTTG immunoreactive co-localizing with 5-bromo-2'-deoxyuridine (BrdU) in the hepatocyte nucleus was found and there was a concurrent sister chromatin itself by the immunofluorescent labeling of PTTG with cytokeratin 18 (CK18).
Discussion: Since the correlation of PTTG mRNA expression, cell proliferation and immunoreactivity were observed in primary rat cultured hepatocytes, PTTG may be a novel marker of cell proliferation both in vitro and in vivo liver regeneration.
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| http://dx.doi.org/10.1016/j.jss.2005.04.019 | DOI Listing |
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