Purpose: Cryopreserved human blood vessels may become important tools in bypass surgery. Optimal cryopreservation of an arterial graft should, therefore, preserve both histological and physiological characteristics of smooth muscle and endothelium comparable to the unfrozen artery.
Methods: Rings from human internal mammary arteries (IMA) were investigated in vitro either unfrozen or after immersion into a cryomedium (RPMI 1640 containing 1.8M Me2SO and 0.1M sucrose) and cryostorage with and without surrounding medium.
Results: In unfrozen IMA, neither contractile responses to noradrenaline (NA) nor endothelium-dependent relaxant responses to acetylcholine (ACH) was modified after exposure of the IMA to cryomedium or during activation of protein kinase C by phorbol-12,13-dibutyrate (PDBu). Exposure to cryomedium with gradually increasing Me2SO content before starting the cooling process did not improve the post-thaw functional activity of the artery. Optimal post-thaw recovery of contractile responses to NA and PGF(2alpha) was observed after freezing at a speed of -1.2 and -3 degrees C/min in arteries stored with and without surrounding cryomedium. Compared to unfrozen controls, the ACH-induced endothelium-dependent relaxation during active tone induced by 3 microM PGF(2alpha) reached 16 and 56% after freezing with and without surrounding medium. All functional data were reflected by electron microscopy images showing considerably better preservation of the endothelial layer after freezing without medium.
Conclusion: Freezing of human arteries at a mean cooling rate of -3 degrees C/min and storage without surrounding medium offers the prospect of optimal preservation of both smooth muscle and endothelial function in cryopreserved human IMA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cryobiol.2005.04.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Application of cryoablation in animal models of bladder and muscle-invasive bladder cancer: a narrative review of current status and future perspectives.
Quant Imaging Med Surg
December 2024
Department of Minimally Invasive Tumor Therapies Center, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, and Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Background And Objective: In patients diagnosed with bladder cancer (BC), muscle-invasive bladder cancer (MIBC) accounts for 25%, including tumors invading the intrinsic muscle layer (T2), surrounding tissues of the bladder (T3), or adjacent pelvic organs/structures (T4). Cryoablation promises to be a novel interventional oncology technique, which effectively induces cellular destruction through freezing and thawing cycles. In addition to the direct tumor-killing effect, it also has a tumor-immune effect.
View Article and Find Full Text PDFBlock cationic copolymer/quaternary ammonium chitosan-based composite antibacterial hydrogel dressings with NIR photothermal effects for bacteria-infected wound healing.
Int J Biol Macromol
December 2024
School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, PR China. Electronic address:
Wound infections caused by pathogenic bacteria can cause delayed wound healing and even threaten the life of patients. Drug resistance and effectiveness with regard to traditional wound dressings have become urgent issues which need to be addressed. The antibacterial effect of hydrogels, as novel wound dressings, is through contact-active antibacterial was limited by the contact area and time.
View Article and Find Full Text PDFDeforming Ice with Drops.
Phys Rev Lett
November 2024
Physics of Fluids group, Max Planck Center Twente for Complex Fluid Dynamics, Department of Science and Technology, Mesa+ Institute and J. M. Burgers Center for Fluid Dynamics, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
A uniform solidification front undergoes nontrivial deformations when encountering an insoluble dispersed particle in a melt. For solid particles, the overall deformation characteristics are primarily dictated by heat transfer between the particle and the surrounding, remaining unaffected by the rate of approach of the solidification front. In this Letter we show that, conversely, when interacting with a droplet or a bubble, the deformation behavior exhibits entirely different and unexpected behavior.
View Article and Find Full Text PDFA Polyvinyl Alcohol (PVA)-Based Phantom for Prostate Cancer Detection Using Multiparametric Ultrasound: A Validation Study.
Bioengineering (Basel)
October 2024
Division of Imaging Sciences and Technology, School of Medicine, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, UK.
Unlabelled: Multiparametric ultrasound (mpUS) enhances prostate cancer (PCa) diagnosis by using multiple imaging modalities. Tissue-mimicking materials (TMM) phantoms, favoured over animal models for ethical and consistency reasons, were created using polyvinyl alcohol (PVA) with varying molecular weights (Mw).
Methods: Four PVA samples, varying in Mw with constant concertation, were mixed with glycerol, silicon carbide (SiC), and aluminium oxide (AlO).
Subretinal Amniotic Membrane Transplantation in a Porcine Model of Retinal Hole.
Invest Ophthalmol Vis Sci
November 2024
Department of Ophthalmology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Purpose: To investigate the histopathological changes following subretinal amniotic membrane transplantation in an in vivo porcine model of retinal holes.
Methods: Left eyes of 12 Danish Landrace pigs were vitrectomized under full anesthesia. A subretinal bleb was created before excising a retinal hole (1154-2934 µm) using a 23-gauge vitrector.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!