Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1151830 | PMC |
http://dx.doi.org/10.1128/AEM.71.6.3359-3363.2005 | DOI Listing |
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