Natural killer T (NKT) cells are a unique T-cell population that is positively selected by CD1d-expressing cells. In this study, we examined the kinetics of conventional CD4+TCRbeta+ and CD4-TCRbeta+ cells along with various NKT cell populations from WT and CD1d KO mice after oral Listeria monocytogenes (Lm) infection at different time points in tissue compartments. We found that CD4+TCRbeta+ cells expressing NK1.1+ (NKT) were constitutively expressed in the lung of both strains of mice, but disappeared after infection. In contrast, CD4-TCRbeta+ NK1.1+ cells migrated to the spleen. Here, we demonstrated that endogenous IL-12 was predominantly expressed in the spleen of CD1d KO mice 2 days after infection, whereas IL-4 was predominantly expressed in the liver of WT mice. Higher levels of IFN-gamma were expressed in MLN of CD1d KO but not in WT mice on day 5. Thus, tissue-specific ligands orchestrate the localization and activation of NKT cells to control immune response to Listeria, which may explain the difference in disease susceptibility.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cellimm.2005.01.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pulchinenoside B4 alleviates DSS-induced colitis by inhibiting CD1d-dependent NLRP3 inflammasome activation in macrophages.
Int Immunopharmacol
January 2025
Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, China. Electronic address:
Ulcerative colitis (UC) represents a significant challenge to global health, underscoring the importance of developing novel alternative anti-colitis agents. Inhibition of the NLRP3 inflammasome in macrophages has emerged as a potential therapeutic strategy for UC. Pulchinenoside B4 (PB4) is a major component of traditional medicinal plants that demonstrated to possess promising anti-inflammatory properties.
View Article and Find Full Text PDFThe α glycolipid rules the NKT cell TCR.
J Exp Med
February 2025
La Jolla Institute for Immunology, La Jolla, CA, USA.
In this issue of JEM, Hosono et al. (https://doi.org/10.
View Article and Find Full Text PDFIdentification of α-galactosylceramide as an endogenous mammalian antigen for iNKT cells.
J Exp Med
February 2025
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Article Synopsis
- Invariant natural killer T (iNKT) cells are unique T cells that recognize lipid antigens through a molecule called CD1d, with α-galactosylceramide (α-GalCer) being the strongest known antigen.
- Researchers created a specialized system using supercritical fluid chromatography tandem mass spectrometry (SFC/MS/MS) to separate and identify different forms of hexosylceramide.
- Their findings revealed that α-GalCer, previously not found in mammals, is present in various biological fluids, representing the first identification of this potent antigen in mammalian systems.
State of play in the molecular presentation and recognition of anti-tumor lipid-based analogues.
Front Immunol
December 2024
Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
The Natural Killer T cells (NKT) are a unique subset of T lymphocytes that recognize lipid-based antigens that are presented by the monomorphic MHC-I-like molecule, CD1d. Over 30 years ago, the discovery of the glycolipid α-Galactosylceramide (α-GalCer) from the marine sponge , as a potent activator of the invariant Natural Killer T (iNKT) cells, has attracted great attention for its use in cancer immunotherapy. However, α-GalCer can initiate both pro-inflammatory T helper cell 1 (Th1) and anti-inflammatory Th2 type immune responses that can result in either enhanced or suppressed immunity in a somewhat unpredictable manner.
View Article and Find Full Text PDFHighly Selective Cytokine Induction of Nitrated Lipid-Modified α-GalCer Derivatives Demonstrating High Binding Affinity to the Lipid Antigen Presenting Molecule CD1d.
Chemistry
January 2025
Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
Article Synopsis
- - Glycolipid antigens are recognized by CD1d on antigen-presenting cells and trigger immune responses in NKT cells through cytokine release.
- - The study discovered a specific glycolipid (α-GalCer nitro-type) that selectively induces Th2 and Th17 cytokines and has a strong binding affinity to CD1d due to modified fatty acyl groups.
- - The introduction of natural nitroalkene groups in these glycolipids enhances their interaction with CD1d, which helps explain their unique role in cytokine induction and selectivity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!